Duan Shu-Rong, Wang Hui-Hui, Qi Ji-Ping, Teng Wen-Li, Xu Ran, Wang De-Sheng, Wang Hai-Tao
Department of Neurology, the First Affiliated Hospital of Harbin Medical Colleges, Harbin 150001, China.
Zhonghua Yi Xue Za Zhi. 2008 Dec 23;88(47):3337-41.
To investigate the relationship of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) and proliferation of endogenous neural stem cells (NSCs) after human cerebral infarction.
Paraffin-embedded brain tissues of 22 human fatal cases of CI from the brain tissues around subventricular zone and subgranular layer zone were stained with HE and immunohistochemistry stain. The endogenous neural stem cells were marked by nestin. The expression changes of EGF, bFGF and nestin in the perihematomal tissues were analysed with the SPSS 13.0 system.
(1) Compared with the controls, the number of nestin-positive cells increased at 24 - 72 h (14 +/- 6)/HP in the ipsilateral SVZ and began to rise at 4.5 - 10 h (11 +/- 5)/HP in the ipsilateral SGZ, reached maximum at 120 - 144 h ((38 +/- 7)/HP in the SVZ, (54 +/- 17)/HP in the SGZ, and decreased markedly at 216 - 336 h, but it was still elevated compared with the controls (P < 0.05). (2) The number of bFGF-positive cells increased at 4.5 - 10 h (8.1 +/- 2.9)/HP in the SVZ, (19.0 +/- 8.2)/HP in the SGZ, reached maximum at 24 - 70 h (15.6 +/- 3.5)/HP in the SVZ, (32.0 +/- 5.7)/HP in the SGZ and decreased at 72 - 96 h, but it was still elevated compared with the controls (P < 0.05). (3) The number of EGF-positive cells increased at 4.5 - 10 h (4.3 +/- 1.6)/HP in the SVZ, (7.0 +/- 3.7)/HP in the SGZ, reached maximum at 120 - 144 h (27.0 +/- 1.4)/HP in the SVZ, (51.5 +/- 4.9)/HP in the SGZ and decreased at 216 - 336 h, but it was still elevated compared with the controls (P < 0.05).
Perhaps the increased expression of EGF and bFGF after CI was a reaction of endogenous reparation and it correlated with the proliferation and endogenous of neural stem cells in human.
探讨人脑梗死后脑源性神经营养因子（bFGF）和表皮生长因子（EGF）与内源性神经干细胞（NSCs）增殖的关系。
选取22例人脑梗死死亡患者的石蜡包埋脑组织，取脑室下区及颗粒下层周围脑组织，进行苏木精-伊红（HE）染色及免疫组织化学染色。以内巢蛋白标记内源性神经干细胞。采用SPSS 13.0系统分析血肿周围组织中EGF、bFGF及巢蛋白的表达变化。
（1）与对照组相比，同侧脑室下区24～72 h时巢蛋白阳性细胞数增加，为（14±6）/HP，同侧颗粒下层4.5～10 h时开始增加，为（11±5）/HP，120～144 h时达高峰，脑室下区为（38±7）/HP，颗粒下层为（54±17）/HP，216～336 h时明显减少，但仍高于对照组（P<0.05）。（2）bFGF阳性细胞数在脑室下区4.5～10 h时增加，为（8.1±2.9）/HP，颗粒下层为（19.0±8.2）/HP，24～70 h时达高峰，脑室下区为（15.6±3.5）/HP，颗粒下层为（32.0±5.7）/HP，72～96 h时减少，但仍高于对照组（P<0.05）。（3）EGF阳性细胞数在脑室下区4.5～10 h时增加，为（4.3±1.6）/HP，颗粒下层为（7.0±3.7）/HP，120～144 h时达高峰，脑室下区为（27.0±1.4）/HP，颗粒下层为（51.5±4.9）/HP，216～336 h时减少，但仍高于对照组（P<0.05）。
脑梗死后脑源性神经营养因子和表皮生长因子表达增加可能是内源性修复反应，与人神经干细胞的增殖及内源性有关。
