Li Ying, Umasankar Yogeswaran, Chen Shen-Ming
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1 Section 3 Chung-Hsiao East Road, Taipei, Taiwan, ROC.
Anal Biochem. 2009 May 15;388(2):288-95. doi: 10.1016/j.ab.2009.02.032. Epub 2009 Mar 1.
A novel biocomposite film (MWCNTs-PNDGAChi), which contains multiwalled carbon nanotubes (MWCNTs) along with the incorporation of poly(nordihydroguaiaretic acid) and chitosan copolymer (PNDGAChi), has been synthesized on gold electrode by potentiostatic methods. The presence of MWCNTs in the biocomposite film enhances PNDGAChi's surface coverage concentration (Gamma) on the electrode and decreases degradation of PNDGAChi during cycling. The biocomposite film also exhibits promising enhanced electrocatalytic activity toward the oxidation of biochemical compounds such as epinephrine (EP) and norepinephrine (NEP). Cyclic voltammetry was used for the measurement of electroanalytical properties of analytes by means of MWCNTs-PNDGAChi biocomposite film modified gold electrode. The sensitivity values of MWCNTs-PNDGAChi biocomposite film modified gold electrode are higher than the values obtained for PNDGAChi film modified gold electrode. Electrochemical quartz crystal microbalance studies reveal the enhancements in the functional properties of MWCNTs and PNDGAChi present in MWCNTs-PNDGAChi biocomposite film. Surface morphology of the biocomposite films was studied using scanning electron microscopy, atomic force microscopy, and scanning tunneling microscopy. The surface morphology results reveal that PNDGAChi incorporated on MWCNTs. Finally, flow injection analysis was used for the amperometric detection of EP and NEP at MWCNTs-PNDGAChi film modified screen printed carbon electrode.
一种新型生物复合膜（MWCNTs-PNDGAChi）已通过恒电位法在金电极上合成，该膜包含多壁碳纳米管（MWCNTs）以及聚（去甲二氢愈创木酸）和壳聚糖共聚物（PNDGAChi）。生物复合膜中MWCNTs的存在提高了PNDGAChi在电极上的表面覆盖浓度（Gamma），并减少了循环过程中PNDGAChi的降解。该生物复合膜还对肾上腺素（EP）和去甲肾上腺素（NEP）等生化化合物的氧化表现出有前景的增强电催化活性。循环伏安法用于通过MWCNTs-PNDGAChi生物复合膜修饰的金电极测量分析物的电分析性质。MWCNTs-PNDGAChi生物复合膜修饰的金电极的灵敏度值高于PNDGAChi膜修饰的金电极所获得的值。电化学石英晶体微天平研究揭示了MWCNTs-PNDGAChi生物复合膜中MWCNTs和PNDGAChi功能特性的增强。使用扫描电子显微镜、原子力显微镜和扫描隧道显微镜研究了生物复合膜的表面形态。表面形态结果表明PNDGAChi结合在了MWCNTs上。最后，流动注射分析用于在MWCNTs-PNDGAChi膜修饰的丝网印刷碳电极上对EP和NEP进行安培检测。
