基于纳米材料传感器的 FAD 依赖酶的生物纳米技术方法。
Bionanotechnology approach for FAD-dependent enzymes with nanomaterials sensor.
机构信息
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
出版信息
Saudi J Biol Sci. 2012 Oct;19(4):465-71. doi: 10.1016/j.sjbs.2012.07.001. Epub 2012 Jul 13.
We have reported the modification of biomolecule with nanomaterials. In this paper, the electrochemical response of different FAD-dependent enzymes at carbon nanomaterials modified electrode. The modified electrode also exhibits a promising enhanced electrocatalytic activity toward the oxidation of substrate. Different methods were used for fabrication of modified electrode. The presence of nanomaterials enhances the enzyme loading and stability. Cyclic voltammograms (CVs) were used for the determination of substrate and the apparent coefficient values for these compounds at different electrodes. Finally, we have studied the surface morphology of the modified electrode using scanning electron microscopy (SEM), which revealed that enzyme is coated on nanomaterials.
我们已经报道了生物分子与纳米材料的修饰。在本文中，我们研究了不同黄素腺嘌呤二核苷酸（FAD）依赖性酶在碳纳米材料修饰电极上的电化学响应。修饰电极对底物的氧化也表现出了有前景的增强电催化活性。我们使用了不同的方法来制备修饰电极。纳米材料的存在增强了酶的负载和稳定性。我们使用循环伏安法（CV）来测定不同电极上的底物和这些化合物的表观系数值。最后，我们使用扫描电子显微镜（SEM）研究了修饰电极的表面形态，结果表明酶覆盖在纳米材料上。
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