Medical Department 1, University Hospital Bergmannsheil, University of Bochum, Germany.
Eur J Med Res. 2009;14(2):65-70. doi: 10.1186/2047-783x-14-2-65.
Disturbances in wound healing in patients with hyperglycaemic blood sugar values are a common clinical problem. Recent studies identified PPARalpha-ligands as potential skin therapeutic agents. The aim of this study was to investigate the effects of oral fenofibrate treatment on dermal wound healing and microcirculatory parameters in diabetic mice.
Dermal wounds were created in CD-1 mice. Mice were randomized into four treatment groups: diabetic mice treated (dbf) or not-treated with fenofibrate (dbnf). As controls served non-diabetic mice treated (ndf) or not-treated with fenofibrate (ndnf). At various points in time microcirculation was analyzed by intravital fluorescent microscopy to determine wound surface area, vessel diameter, plasma leakage, functional capillary density, and leukocyte/endothelium interaction.
The dbf-mice showed a significantly increased diameter of the venules and the arterioles up to 3 days after wound creation compared to dbnf-mice. However, wound healing was not improved in dbf-compared to dbnf-mice. Surprisingly, all microcirculatory parameter (vessel diameter, plasma leakage and functional capillary density) were not deteriorated in dbnf- compared to ndnf-mice.
We confirm that high blood sugar values lead to a delayed wound healing, but this could not traced back to altered microcirculatory patterns. Furthermore, in dbf-mice an improved vasodilatatory function of small vessels could be detected, but with no substantial effect on wound healing. Further studies are needed to clarify, if topical application of fenofibrate might be beneficial.
高血糖患者的伤口愈合障碍是一个常见的临床问题。最近的研究发现过氧化物酶体增殖物激活受体-α配体是有潜力的皮肤治疗药物。本研究旨在探讨口服非诺贝特治疗对糖尿病小鼠皮肤伤口愈合和微循环参数的影响。
在 CD-1 小鼠中建立皮肤伤口。将小鼠随机分为四组：糖尿病小鼠用（dbf）或不用（dbnf）非诺贝特治疗。非糖尿病小鼠用（ndf）或不用（ndnf）非诺贝特治疗作为对照。在不同时间点，通过活体荧光显微镜分析微循环，以确定伤口表面积、血管直径、血浆渗漏、功能性毛细血管密度和白细胞/内皮细胞相互作用。
dbf 小鼠在伤口形成后 3 天，其小静脉和小动脉的直径明显增大，与 dbf 小鼠相比。然而，dbf 小鼠的伤口愈合并没有比 dbf 小鼠更好。令人惊讶的是，dbnf 小鼠的所有微循环参数（血管直径、血浆渗漏和功能性毛细血管密度）均未恶化与 ndnf 小鼠相比。
我们证实高血糖值会导致伤口愈合延迟，但这不能归因于微循环模式的改变。此外，在 dbf 小鼠中可以检测到小血管的血管扩张功能得到改善，但对伤口愈合没有实质性影响。需要进一步的研究来阐明，局部应用非诺贝特是否有益。
