Hermida Jesús, Tutor J Carlos
Unidad Monitorización Fármacos, Laboratorio Central, Hospital Clínico Universitario, Santiago de Compostela, Spain.
Ther Drug Monit. 2009 Apr;31(2):269-72. doi: 10.1097/FTD.0b013e31819c6d5c.
The presence of endogenous antibodies in the serum of some patients has long been known to be a potential source of interference in immunoassays. We report falsely increased whole blood tacrolimus concentrations using the antibody conjugated magnetic immunoassay from Siemens HealthCare Diagnostics in a liver transplant recipient due to the presence of circulating endogenous antibodies (possibly heterophilic antibodies). Estimation of whole blood tacrolimus concentrations from the washed erythrocytes concentrations is proposed as a tentative approach for obtaining reliable results using the antibody conjugated magnetic immunoassay assay in these cases, leading to analogous blood tacrolimus concentrations to those produced by the microparticle enzyme immunoassay from Abbott Laboratories.
长期以来，人们一直知道某些患者血清中存在内源性抗体是免疫测定中潜在的干扰源。我们报告了一名肝移植受者因循环内源性抗体（可能是嗜异性抗体）的存在，使用西门子医疗诊断公司的抗体偶联磁免疫测定法时全血他克莫司浓度出现假性升高。在这些情况下，建议从洗涤后的红细胞浓度估算全血他克莫司浓度，作为使用抗体偶联磁免疫测定法获得可靠结果的一种尝试性方法，其得出的血他克莫司浓度与雅培实验室的微粒酶免疫测定法得出的浓度相似。
