Gui Fei, Ma Wei-Feng, Cai Shao-Hui, Li Xiao-Kun, Tan Yi, Zhou Chun-Ling, Chen Hong-Yuan
Pharmacology Laboratory Center, Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2008 Nov;31(11):1685-9.
To investigate the effects of curcumine on mouse B16 melanoma growth and possible mechanism of Bcl-2, P53 and glutathione in tumor cells.
The inhibitory effect on growth of melanoma in vivo were examined by mice melanoma models transplanted B16 cells to C57BL/6J mice. MTT method was used to assay the contribution of curcumine to B16 cells in vitro. The apoptosis and expression of Bcl-2, P53 gene of B16 cells were analyzed by flow cytometry, and HPLC assay was used to detect the change of GSH in B16 melanom tissues of C57BL/6J mouse caused by curcumine.
Curcumine had obvious inhibitory effect on the growth of mouse B16 melanoma in time and dose dependent manner and the gene expression of bcl-2 in B16 cells decreased after 24 hours supplied with curcumine, whereas P53 protein expression increased; Curcumine depressed the GSH quantity in melanoma tissues.
The growth inhibitory effect of curcumine on mouse melanom is proved in vivo and in vitro respectively. Curcumine can induce some cells to apoptosis which may be relevant to downregulation of bcl-2 expression and upregulation of P53 expression as well as exhaustion of GSH in tumor organization.
探讨姜黄素对小鼠B16黑色素瘤生长的影响以及肿瘤细胞中Bcl-2、P53和谷胱甘肽的可能作用机制。
通过将B16细胞移植到C57BL/6J小鼠建立小鼠黑色素瘤模型，检测姜黄素对体内黑色素瘤生长的抑制作用。采用MTT法检测姜黄素对体外B16细胞的作用。通过流式细胞术分析B16细胞的凋亡及Bcl-2、P53基因的表达，并采用高效液相色谱法检测姜黄素对C57BL/6J小鼠B16黑色素瘤组织中谷胱甘肽的影响。
姜黄素对小鼠B16黑色素瘤的生长具有明显的抑制作用，呈时间和剂量依赖性；给予姜黄素24小时后，B16细胞中bcl-2的基因表达下降，而P53蛋白表达增加；姜黄素降低了黑色素瘤组织中的谷胱甘肽含量。
分别在体内和体外证实了姜黄素对小鼠黑色素瘤生长的抑制作用。姜黄素可诱导部分细胞凋亡, 这可能与肿瘤组织中bcl-2表达下调、P53表达上调以及谷胱甘肽耗竭有关。
