Guo Juan, Chen Chang-Xun, Du Jun, Gu Wei-Liang, Shen Yun-Hui
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Yao Cai. 2008 Nov;31(11):1691-5.
To investigate the effects of Semen descurainiae and Captopril on CYP11B1, CYP11B2 and TGF-beta1 mRNA expression of heart tissue in rats treated with Abdominal Aortic Banding.
Ventricular remodeling was induced by abdominal aortic banding (AAB) in rats. After 30 days' treatment, the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; Then the CYP11B, CYP11B2 and TGF-beta1 mRNA expression of left ventricle were detected by Real-time PCR, respectively.
The experimental data demonstrated that Semen descurainiae decreased the indexes of LVW/BW and HW/BW, down-regulated CYP11B, CYP11B2 and TGF-beta1 mRNA expression in left ventricle (P<0.05).
Semen desceurainiae can significantly inhibit the experimental ventricular remodeling; the mechanism is related to its ability to attenuate the mRNA expression of CYP11B1, CYP11B2 and TGF-beta1 in left ventricle. The inhibition of aldosterone key gene expression by Semen descurainiae may contribute to its effect on restraint cardiac remodeling.
探讨播娘蒿和卡托普利对腹主动脉缩窄大鼠心脏组织CYP11B1、CYP11B2和转化生长因子β1（TGF-β1）mRNA表达的影响。
采用腹主动脉缩窄法（AAB）诱导大鼠心室重构。治疗30天后，计算左心室重量/体重（LVW/BW）、心脏重量/体重（HW/BW）比值；然后分别采用实时荧光定量PCR检测左心室CYP11B1、CYP11B2和TGF-β1 mRNA表达。
实验数据表明，播娘蒿降低了LVW/BW和HW/BW指标，下调了左心室CYP11B1、CYP11B2和TGF-β1 mRNA表达（P<0.05）。
播娘蒿可显著抑制实验性心室重构；其机制与其降低左心室CYP11B1、CYP11B2和TGF-β1 mRNA表达有关。播娘蒿对醛固酮关键基因表达的抑制作用可能是其抑制心脏重构的作用机制之一。
