Guo Juan, Chen Chang-xun
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Yao Cai. 2007 Aug;30(8):963-7.
To approach the influnce of Tinglizi on some neuroendocrine factors and type I and III collagen in ventricular remodeling induced by Abdominal Aortic Banding (AAB) in rats.
Myocardial hypertrophy ventricular remodeling model was induced by abdominal aortic banding (AAB) in rats. After 30 day treatment, the systolic blood pressure (SBP) diastolic blood pressure (DBP) were measured; Then the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; The Angiotensin II (Ang II) and Endothelin (ET-1) content in heart tissue and the Aldosterone (ALD) concentration of blood plasma were determined by radioimmunoassay. The content of type I and III collagen in myocardium were determined using immunohistochemical analysis. Hydroxy proline content in heart tissue was measured by hydrolysis method.
The experimental data demonstrated that Tinglizi could decrease SBP, DBP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of ANg II, ALD and ET, decrease the total collagen content and type I and III collagen (P<0.05).
Tiglizi can significantly attenuate the experimental ventricular remodeling; the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system (RAAS) and systema nervosum sympatheticum (SNS), decrease the content of neuroendocrine factors (Ang II , ET, ALD).
探讨葶苈子对腹主动脉缩窄（AAB）诱导的大鼠心室重构中某些神经内分泌因子及Ⅰ型和Ⅲ型胶原的影响。
采用腹主动脉缩窄（AAB）法诱导大鼠心肌肥厚心室重构模型。治疗30天后，测量收缩压（SBP）、舒张压（DBP）；计算左心室重量/体重（LVW/BW）、心脏重量/体重（HW/BW）比值；采用放射免疫分析法测定心脏组织中血管紧张素Ⅱ（AngⅡ）和内皮素（ET-1）含量及血浆醛固酮（ALD）浓度。采用免疫组织化学分析法测定心肌中Ⅰ型和Ⅲ型胶原含量。采用水解法测定心脏组织中羟脯氨酸含量。
实验数据表明，葶苈子可降低SBP、DBP及LVW/BW和HW/BW的心脏指数，显著降低AngⅡ、ALD和ET含量，降低总胶原含量及Ⅰ型和Ⅲ型胶原含量（P<0.05）。
葶苈子可显著减轻实验性心室重构；其机制与其抑制肾素-血管紧张素-醛固酮系统（RAAS）和交感神经系统（SNS）激活、降低神经内分泌因子（AngⅡ, ET, ALD）含量有关。
