Liu Yi-hui, Chen Bin-long, Zhou Xiao-long, Li Pan, Qiu Ying-xiong, Fu Cheng-xin
Laboratory of Plant Systematic Evolution and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2756-60.
To investigate the genetic diversity of main germplasm of Atractylodes macrocephala in China and the genetic differentiation of the germplasm of A. macrocephala.
A molecular marker ISSR was used to analyze the genetic diversity of 7 populations of A. macrocephala and a population of A. lancea.
Twelve primers were used in the PCR amplification of 86 samples of A. macrocephala and 5 samples of A. lancea. Sixty-three bands with sizes ranged from 100 to 2500 bp were generated from 12 primers. Of all the 63 bands, 55 bands were polymorphic among 86 individuals of A. macrocephala, the percentage of polymorphic bands were 87.30% at the species level. The percentage of polymorphic bands (PPL) for a single population ranged from 58.73% to 71.43% (mean, 64.85%). Among the 7 populations, a population from Panan, GM exhibited highest variability (PPL =71.43%; HE = 0.2835; I = 0.4267). A dendrogram constructed by an unweighted pair group method of cluster analysis showed that populations from Panan constructed one branch and separated from other populations. In the AMOVA analysis, low level of genetic differentiation among populations was detected, 90.52% of the variability existed in population.
The genetic diversity of cultivated A. macrocephala in China is high, which is good for the production of high quality herb medicine.
研究中国白术主要种质的遗传多样性及白术种质的遗传分化。
采用分子标记ISSR对7个白术居群和1个苍术居群进行遗传多样性分析。
用12条引物对86份白术样品和5份苍术样品进行PCR扩增，共产生63条大小在100～2500 bp之间的条带。在这63条条带中，86份白术个体间有55条具有多态性，种水平多态性条带百分率为87.30%。单个居群的多态性条带百分率（PPL）在58.73%～71.43%之间（平均为64.85%）。7个居群中，磐安的一个居群GM变异性最高（PPL =71.43%；HE = 0.2835；I = 0.4267）。通过非加权配对组法聚类分析构建的树状图显示，磐安的居群形成一个分支并与其他居群分开。在AMOVA分析中，检测到居群间遗传分化水平较低，90.52%的变异存在于居群内。
中国栽培白术遗传多样性高，有利于优质药材生产。
Zotero 插件