Sun Liang, Zheng Yu-Guang, Yuan Yuan, Bu Jing, Wang Xue, Jiang Chao, Chen Min
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2012 Nov;37(22):3381-5.
To assess the genetic diversity of Atractylodes macrocephala in 12 cultivated populations and 3 wild populations.
The molecular marker technique ISSR was used to investigate the genetic diversity of 365 samples from 15 populations in Zhejiang, Anhui and Hebei Provinces. Data was analyzed by POPGEN 32, and a cluster diagram was presented by UPGMA.
One hundred and two amplified fragments were obtained using 13 ISSR primers. 94 polymorphic loci were identified as 92.16%. Nei's genetic diversity index (He) was 0.406 5, Shannon diversity index (I) was 0.590 3, and the coefficient of gene differentiation (Gst) was 0.202 5. The genetic similarity coefficient among the populations ranged from 0.690 7 to 0.960 5 in an average of 0. 825 6. No significant difference was found among the populations based on the clustering analysis.
There is a low level of genetic differentiation in higher genetic diversity of the A. macrocephala populations. No obvious regional or species diversities were identified. This is consistent with current status of cultivation and gerplasm circulation in A. macrocephala.
评估12个栽培居群和3个野生居群白术的遗传多样性。
采用分子标记技术ISSR对浙江、安徽和河北3省15个居群的365份样品进行遗传多样性研究。数据经POPGEN 32分析,并采用UPGMA构建聚类图。
13条ISSR引物共扩增出102条片段,其中多态性位点94个,多态位点百分率为92.16%。Nei氏遗传多样性指数(He)为0.406 5,Shannon多样性指数(I)为0.590 3,基因分化系数(Gst)为0.202 5。居群间遗传相似系数范围为0.690 7至0.960 5,平均为0.825 6。聚类分析结果显示居群间无显著差异。
白术居群遗传多样性较高,但遗传分化水平较低。未发现明显的区域或种源差异。这与白术目前的栽培及种质流通现状相符。