Zhong Shan, Yang De-po, Cui Zheng
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2781-4.
To study the anticoagulant constituents in dried leech (Whitmania pigra Whitman).
The plasma recalcification time (PRT) as index, the constituents with anticoagulant activity were isolated and purified by anion-exchange chromatography on Sephadex DEAE A-50, gel permeation chromatography on Sephadex G-25 and Sephadex LH-20 columns, and then reversed phase high-performance liquid chromatography successively.
Three anticoagulant polypeptides were isolated and purified. Compounds 1 and 2 can be translated each other in natural conditions, and their molecular weights are 7100 and 5531, respectively. Compound 3 was identified as a pure polypeptide by HPLC and SDS-PAGE, and its molecular weight was determined as 8 608 by MALDI-TOF-MS. The amino acid composition of compound 3 was also determined.
Compound 3 was inferred to be a novel anticoagulant, and named whitmanin.
研究宽体金线蛭（Whitmania pigra Whitman）干燥品中的抗凝血成分。
以血浆复钙时间（PRT）为指标，先后通过Sephadex DEAE A - 50阴离子交换色谱、Sephadex G - 25和Sephadex LH - 20柱凝胶渗透色谱以及反相高效液相色谱对具有抗凝血活性的成分进行分离纯化。
分离纯化得到三种抗凝血多肽。化合物1和2在自然条件下可相互转化，其分子量分别为7100和5531。化合物3经高效液相色谱（HPLC）和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）鉴定为纯多肽，基质辅助激光解吸电离飞行时间质谱（MALDI - TOF - MS）测定其分子量为8608，并测定了化合物3的氨基酸组成。
推断化合物3为一种新型抗凝血剂，命名为水蛭素。
