Kantachote Duangporn, Charernjiratrakul Wilawan
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat-Yai 90112, Thailand.
Pak J Biol Sci. 2008 Nov 15;11(22):2545-52. doi: 10.3923/pjbs.2008.2545.2552.
Lactic Acid Bacteria (LAB) from fermented plant beverages were selected based on their antibacterial actions against potential food borne pathogenic bacteria (Staphylococcus aureus PSSCMI 0004, Escherichia coli PSSCMI 0001, Salmonella typhimurium PSSCMI 0034 and Vibrio parahaemolyticus VP 4). Antibacterial activities were measured using an agar spot method. The Lactobacillus plantarum W90A strain isolated from a wild forest noni (Morinda coreia Ham) beverage was used as an inoculant. Three different inoculation procedures were conducted with the fruit of wild forest noni fermentations to establish which one was the best for controlling the numbers of yeast in the finished product. A 5% inoculum of L. plantarum W90A (LAB set), initial cell density 8.6 log cfu mL(-1), produced a better product and inhibitory properties against the test organisms, particularly E. coli PSSCMI 0001 than one with no inoculum or with a 5% inoculum from a previous natural fermented product. An LAB inoculum resulted in a reduced total bacterial count and no yeast throughout fermentation period (90 days). The lower yeast resulted in a reduction of the ethanol content to 2.9 g L(-1) compared to 12.2 g L(-1) inthe culture with no inoculum. The highest acidity (1.3-1.4%) with the same pH (3.3) was observed in both sets of inoculated fermentations, whereas the uninoculated set gave a pH value of 3.7 (1.2% acidity).
基于对潜在食源性病原体（金黄色葡萄球菌PSSCMI 0004、大肠杆菌PSSCMI 0001、鼠伤寒沙门氏菌PSSCMI 0034和副溶血性弧菌VP 4）的抗菌作用，从发酵植物饮料中筛选乳酸菌（LAB）。采用琼脂斑点法测定抗菌活性。从野生森林诺丽（海滨巴戟天）饮料中分离出的植物乳杆菌W90A菌株用作接种物。对野生森林诺丽发酵果实进行了三种不同的接种程序，以确定哪种程序最适合控制成品中的酵母数量。接种5%的植物乳杆菌W90A（LAB组），初始细胞密度为8.6 log cfu mL(-1)，与未接种或接种5%先前天然发酵产物接种物的情况相比，产生了更好的产品，对测试微生物，特别是大肠杆菌PSSCMI 0001具有抑制特性。LAB接种物导致总细菌数减少，并且在整个发酵期（90天）内没有酵母。与未接种培养物中12.2 g L(-1)的乙醇含量相比，较低的酵母含量导致乙醇含量降至2.9 g L(-1)。在两组接种发酵中观察到相同pH值（3.3）下的最高酸度（1.3 - 1.4%），而未接种组的pH值为3.7（酸度1.2%）。
