Breed Dana R, Thibault Raymond, Xie Fang, Wang Qian, Hawker Craig J, Pine David J
Center for Soft Matter Research, Department of Physics, New York University, 4 Washington Place, New York, New York 10003, USA.
Langmuir. 2009 Apr 21;25(8):4370-6. doi: 10.1021/la801880u.
We describe a new method to covalently link a wide variety of molecules to the surface of colloidal polymer microspheres using the Cu(I)-catalyzed azide-alkyne reaction, most commonly known under the class of reactions identified by the term click chemistry. The method is generic and readily applied to a spectrum of colloidal particle systems allowing surfaces to be tailored with virtually any desired functionality. To demonstrate this method, polystyrene microspheres were functionalized with two different polyethylene oxide-based polymers, and changes in hydrodynamic radii after functionalization were measured using dynamic light scattering. Control of surface functional groups was demonstrated by fluorescently labeling the colloidal microspheres using the same Cu-catalyzed azide-alkyne cycloaddition reaction.
我们描述了一种使用铜（I）催化的叠氮化物-炔烃反应将多种分子共价连接到胶体聚合物微球表面的新方法，该反应在术语“点击化学”所定义的反应类别中最为常见。该方法具有通用性，可轻松应用于一系列胶体颗粒系统，使表面能够用几乎任何所需的功能进行定制。为了证明该方法，用两种不同的基于聚环氧乙烷的聚合物对聚苯乙烯微球进行功能化，并使用动态光散射测量功能化后流体动力学半径的变化。通过使用相同的铜催化叠氮化物-炔烃环加成反应对胶体微球进行荧光标记，证明了对表面官能团的控制。
