Yamashita H, Mori K, Nakai T
Ehime Prefectural Fisheries Experimental Station, Uwajima, Ehime, Japan.
J Fish Dis. 2009 Feb;32(2):201-10. doi: 10.1111/j.1365-2761.2008.01017.x.
An aquabirnavirus (ABV) and a formalin-inactivated betanodavirus [redspotted grouper nervous necrosis virus (RGNNV)] were investigated for their potential to prevent RGNNV-induced viral nervous necrosis (VNN) in the sevenband grouper, Epinephelus septemfasciatus (Thunberg). Three groups of fish were injected intramuscularly with ABV, intraperitoneally with inactivated RGNNV (iRGNNV) or with both ABV and iRGNNV. At 3, 7, 14, 21 and 28 days post-injection (p.i.), fish were challenged by intramuscular injection of RGNNV. Control fish, which received neither ABV nor iRGNNV, showed high mortalities in all RGNNV challenges. Fish that received only ABV exhibited relative percent survival (RPS) of >60 against RGNNV challenges at 3, 7, 14 and 21 days p.i., but not at 28 days p.i., while fish that received only iRGNNV showed significantly higher protection against RGNNV challenges only at 21 and 28 days p.i. In contrast, fish that received both ABV and iRGNNV showed 60 or higher RPS against all RGNNV challenges. Fish inoculated with iRGNNV with or without ABV exhibited similar high titres of neutralizing antibodies to RGNNV at 14, 21 and 28 days p.i. These results indicate that combined inoculation with iRGNNV and ABV conferred both rapid non-specific and delayed specific protection against VNN.
研究了一种水生双RNA病毒（ABV）和一种福尔马林灭活的β-诺达病毒[红斑石斑鱼神经坏死病毒（RGNNV）]预防七带石斑鱼（Epinephelus septemfasciatus，Thunberg）感染RGNNV所致病毒性神经坏死（VNN）的潜力。将三组鱼分别肌肉注射ABV、腹腔注射灭活RGNNV（iRGNNV）或同时注射ABV和iRGNNV。在注射后3、7、14、21和28天，通过肌肉注射RGNNV对鱼进行攻毒。未接受ABV和iRGNNV的对照鱼在所有RGNNV攻毒中均表现出高死亡率。仅接受ABV的鱼在注射后3、7、14和21天对RGNNV攻毒的相对存活率（RPS）>60%，但在注射后28天未达到，而仅接受iRGNNV的鱼仅在注射后21天和28天对RGNNV攻毒表现出显著更高的保护作用。相比之下，同时接受ABV和iRGNNV的鱼在所有RGNNV攻毒中均表现出60%或更高的RPS。接种了iRGNNV（无论是否接种ABV）的鱼在注射后14、21和28天对RGNNV表现出相似的高滴度中和抗体。这些结果表明，联合接种iRGNNV和ABV可对VNN提供快速的非特异性和延迟的特异性保护。
