Department of Aqualife Medicine, Chonnam National University, Yeosu, Korea.
J Fish Dis. 2012 Mar;35(3):187-91. doi: 10.1111/j.1365-2761.2011.01334.x. Epub 2012 Jan 12.
It was recently reported that Poly(I:C) immunization with live nervous necrosis virus (NNV) confers protection in sevenband grouper, Epinephelus septemfasciatus (Thunberg), from NNV infection. In the present study, we conducted field tests with sevenband grouper for the evaluation of Poly(I:C) immunization efficacy. In the first experiment, sevenband grouper were immunized with NNV followed by Poly(I:C) administration 7 weeks before natural occurrence of viral nervous necrosis (VNN). Survival rate of the naïve fish was 71.0%, whereas that of the immunized fish was 99.8%. In the second experiment, sevenband grouper were immunized 10 months before VNN occurrence and survival rate of the non-treated and vaccinated fish was 79.5% and 97.5%, respectively. In the third experiment, we administered Poly(I:C) to sevenband grouper at 20 days after natural occurrence of VNN. The survival rate of the non-treated fish was 9.8%, whereas that of fish administered Poly(I:C) was 93.7%. Based on these results, it was concluded that Poly(I:C) immunization conferred protection in fish against NNV infection in field tests and the protection lasted more than 10 months. Furthermore, even after occurrence of VNN, fish mortality could be reduced by Poly(I:C) administration and there was an unexpected curative effect on VNN-affected fish.
最近有报道称，用聚肌胞（Poly(I:C)）对携带神经坏死病毒（NNV）的活七带石斑鱼（Epinephelus septemfasciatus）进行免疫接种，可以提供针对 NNV 感染的保护。在本研究中，我们对七带石斑鱼进行了现场试验，以评估聚肌胞免疫接种的效果。在第一个实验中，七带石斑鱼先接种 NNV，然后在自然发生病毒性神经坏死症（VNN）前 7 周进行聚肌胞给药。未处理鱼的存活率为 71.0%，而免疫鱼的存活率为 99.8%。在第二个实验中，七带石斑鱼在 VNN 发生前 10 个月进行免疫接种，未处理和接种疫苗的鱼的存活率分别为 79.5%和 97.5%。在第三个实验中，我们在自然发生 VNN 后 20 天给七带石斑鱼施用聚肌胞。未处理鱼的存活率为 9.8%，而施用聚肌胞的鱼的存活率为 93.7%。根据这些结果可以得出结论，聚肌胞免疫接种在现场试验中对鱼类提供了针对 NNV 感染的保护，而且保护作用持续了 10 个月以上。此外，即使在 VNN 发生后，通过施用聚肌胞也可以降低鱼的死亡率，并且对受 VNN 影响的鱼有意外的治疗效果。
