Gu J, Pinheiro J M, Yu C Z, D'Andrea M, Muralidharan S, Malik A
Deborah Research Institute, Browns Mills, New Jersey 08015-1799.
Tissue Cell. 1991;23(4):437-44. doi: 10.1016/0040-8166(91)90002-b.
We studied tissue sections of freshly obtained full-term and premature human umbilical cords using polyclonal antibody to endothelin and immunocytochemistry. Endothelin immunoreactivity was detected in the cytoplasm of epithelial cells and primitive fibroblasts, but not in the endothelial cells of both full-term and premature umbilical cords. Immunoelectron microscopy using indirect immunogold staining technique localized endothelin immunoreactivity to the cytoplasm of the epithelial cells and fibroblasts but not confined to any particular structures. No endothelin immunoreactivity was detected in the nucleus or on the cell membrane. Pre-absorption tests with synthetic endothelin-1, -2, and -3 independently established that the immunoreactivity represented endothelin-1 and -2, but not -3. The presence of endothelin-1 and -2-like immunoreactive materials in epithelial cells and fibroblasts of human umbilical cord suggests a role of endothelin in parturition.
我们使用抗内皮素多克隆抗体和免疫细胞化学方法，研究了新鲜获取的足月和早产人脐带的组织切片。在足月和早产脐带的上皮细胞和原始成纤维细胞的细胞质中检测到内皮素免疫反应性，但在内皮细胞中未检测到。使用间接免疫金染色技术的免疫电子显微镜将内皮素免疫反应性定位在上皮细胞和成纤维细胞的细胞质中，但不限于任何特定结构。在细胞核或细胞膜上未检测到内皮素免疫反应性。用合成内皮素-1、-2和-3进行的预吸收试验独立证实，该免疫反应性代表内皮素-1和-2，而非-3。人脐带上皮细胞和成纤维细胞中存在内皮素-1和-2样免疫反应性物质，提示内皮素在分娩中起作用。
