Wilkes B M, Susin M, Mento P F
Division of Nephrology and Hypertension, North Shore University Hospital, Manhasset, New York 11030.
J Histochem Cytochem. 1993 Apr;41(4):535-41. doi: 10.1177/41.4.8450193.
We examined the distribution of endothelin-1-like immunoreactivity in human placenta, using the immunoperoxidase technique. A specific polyclonal antibody to endothelin-1 was raised in rabbits, which recognized endothelin-1 and its precursor molecule, big endothelin. Immunoperoxidase staining revealed that endothelin-1-like immunoreactivity was widely distributed in the placenta. Endothelin-1-like immunoreactivity was present in endothelial cells of capillaries of the microvilli and in small- and medium-sized arteries and veins. The distribution of endothelin-1-like immunoreactivity was similar to the distribution of Factor VIII-related antigen, which stains endothelial cells. The nature of endothelin in the human placenta was further examined with cultured umbilical vein endothelial cells. Endothelial cells released endothelin-like material into the culture medium. The amount of endothelin-like material varied directly with time of incubation and the amount of fetal calf serum in the culture medium. Fractionation of the endothelin-1-like material by reversed-phase high-performance liquid chromatography (HPLC) and quantitation by radioimmunoassay (RIA) revealed that endothelin-like immunoreactivity co-eluted with endothelin-1 but not with big endothelin-1. We conclude that endothelin-1-like immunoreactivity is widely distributed in vascular endothelium of the human placenta. These data are compatible with a role for endothelin as an autocrine or paracrine modulator of vascular tone in the human placenta.
我们采用免疫过氧化物酶技术研究了内皮素 -1 样免疫反应性在人胎盘中的分布。用兔制备了一种针对内皮素 -1 的特异性多克隆抗体，该抗体可识别内皮素 -1 及其前体分子大内皮素。免疫过氧化物酶染色显示，内皮素 -1 样免疫反应性在胎盘中广泛分布。内皮素 -1 样免疫反应性存在于微绒毛毛细血管的内皮细胞以及中小动脉和静脉中。内皮素 -1 样免疫反应性的分布与 VIII 因子相关抗原（可对内皮细胞进行染色）的分布相似。我们用培养的脐静脉内皮细胞进一步研究了人胎盘中内皮素的性质。内皮细胞将内皮素样物质释放到培养基中。内皮素样物质的量与孵育时间以及培养基中胎牛血清的量直接相关。通过反相高效液相色谱（HPLC）对内皮素 -1 样物质进行分离，并通过放射免疫测定（RIA）进行定量分析，结果显示内皮素样免疫反应性与内皮素 -1 共洗脱，而与大内皮素 -1 不共洗脱。我们得出结论，内皮素 -1 样免疫反应性广泛分布于人胎盘的血管内皮中。这些数据与内皮素作为人胎盘血管张力的自分泌或旁分泌调节剂的作用相一致。
