高效液相色谱法测定青贮饲料稻中稻曲毒素A的含量
High-performance liquid chromatographic determination of ustiloxin A in forage rice silage.
作者信息
Miyazaki Shigeru, Matsumoto Yuichi, Uchihara Tsutomu, Morimoto Kazuhide
机构信息
Safety Research Team, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, Japan.
出版信息
J Vet Med Sci. 2009 Feb;71(2):239-41. doi: 10.1292/jvms.71.239.
We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column. Separated ustiloxin A was detected with ultraviolet (UV) absorption at 254 nm. The limit of quantitation for ustiloxin A in silage found to be 2.5 mg/kg. The present method can be used for routine monitoring of the contamination of ustiloxin A in forage rice silage.
我们描述了一种用于测定稻曲病菌产生的霉菌毒素——稻曲毒素A在青贮饲料稻青贮中的高效液相色谱方法。将冻干的青贮饲料样品研磨并用水提取。提取物通过固相萃取进行纯化，然后使用十八烷基硅烷键合柱进行高效液相色谱分析。分离出的稻曲毒素A通过在254nm处的紫外（UV）吸收进行检测。发现青贮饲料中稻曲毒素A的定量限为2.5mg/kg。本方法可用于常规监测青贮饲料稻青贮中稻曲毒素A的污染情况。
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