Arciniegas Enrique, Carrillo Luz M, De Sanctis Juan B, Candelle Daniel
Facultad de Medicina, Servicio Autónomo Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela.
Cell Adh Migr. 2008 Jan-Mar;2(1):17-29. doi: 10.4161/cam.2.1.5789. Epub 2008 Jan 23.
The NFkappaB family of transcription factors, particularly the activated p50/p65 heterodimer, is expressed in vascular cells during intimal thickening formation when hemodynamic conditions are altered. Here, we report that p50, p65, IkappaBalpha and IKKalpha display different spatial and temporal patterns of expression and distribution during both chicken embryo aortic wall remodeling and intimal thickening development. Additionally, we show that both p50 and p65 were located in the nucleus of some mesenchymal cells expressing alpha-smooth muscle actin which are present in the spontaneous intimal thickening observed at embryonic days 12-14 of development. We also demonstrated that both NFkappaB subunits are present in monolayers of primary embryonic aortic endothelial cells attached to fibronectin and stimulated with complete medium. This study demonstrates for the first time the presence of activated NFkappaB during the remodeling of the embryonic aortic wall and the formation of intimal thickening, providing evidence that suggest a possible role for this transcription factor in the EndoMT process.
转录因子NFκB家族，尤其是活化的p50/p65异二聚体，在血流动力学条件改变时内膜增厚形成过程中在血管细胞中表达。在此，我们报道在鸡胚主动脉壁重塑和内膜增厚发育过程中，p50、p65、IκBα和IKKα呈现出不同的表达和分布时空模式。此外，我们发现p50和p65都位于一些表达α-平滑肌肌动蛋白的间充质细胞核内，这些间充质细胞存在于发育第12至14天观察到的自发性内膜增厚中。我们还证明，NFκB两个亚基都存在于附着于纤连蛋白并用完全培养基刺激的原代胚胎主动脉内皮细胞单层中。本研究首次证明在胚胎主动脉壁重塑和内膜增厚形成过程中存在活化的NFκB，为该转录因子在内皮-间充质转化过程中可能发挥的作用提供了证据。
