Mikkelsen Michael Dalgaard, Fuller Victoria L, Hansen Bjarne Gram, Nafisi Majse, Olsen Carl Erik, Nielsen Henrik Bjørn, Halkier Barbara Ann
VKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
Planta. 2009 May;229(6):1209-17. doi: 10.1007/s00425-009-0907-5. Epub 2009 Mar 5.
Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
吲哚 - 3 - 乙醛肟（IAOx）是初级代谢和次级代谢之间的关键分支点。IAOx是吲哚硫代葡萄糖苷（I - GLSs）、camalexin和植物激素吲哚 - 3 - 乙酸（IAA）生物合成的中间体。细胞色素P450s CYP79B2和CYP79B3催化色氨酸向IAOx的转化。CYP83B1将IAOx导入I - GLS生物合成，CYP71A13将IAOx导入camalexin生物合成，而IAA生物合成中IAOx代谢酶尚不清楚。在本报告中，我们通过将乙醇（EtOH）诱导型CYP79B2构建体导入双突变体（cyp79b2 cyp79b3）或三突变体（cyp79b2 cyp79b3 cyp83b1）品系，证明了I - GLSs的可控生产。我们展示了camalexin的乙醇依赖性诱导，并通过聚类微阵列分析鉴定了一些与IAA稳态或防御相关的候选基因。因此，转基因突变体系是阐明初级代谢和次级代谢之间相互作用的有前景的工具。
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