Zhu Anwei, Tian Yang, Liu Haiqing, Luo Yongping
Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.
Biomaterials. 2009 Jun;30(18):3183-8. doi: 10.1016/j.biomaterials.2009.02.019. Epub 2009 Mar 5.
A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) - an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (ks) of 3.9s(-1). Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.
报道了一种在透明ITO衬底上制备纳米多孔金膜材料的逐层方法，即通过1,5-戊二硫醇作为交联剂交替组装金和银纳米颗粒，然后在室温下将银纳米颗粒溶解在HAuCl4溶液中。细胞色素c（cyt. c）——一种用于生物分子研究的优秀模型，在纳米多孔金膜上的电子转移得到极大促进，电子转移速率常数（ks）为3.9s(-1)。同时，吸附在纳米多孔金膜上的cyt. c对H2O2仍保持其酶活性。基于这些实验结果，cyt. c-纳米多孔金膜被开发成一种用于H2O2的安培型生物传感器，具有高选择性、宽线性范围、低检测限和长稳定性。
