Khang Dongwoo, Liu-Snyder Peishan, Pareta Rajesh, Lu Jing, Webster Thomas J
Division of Engineering and Department of Orthopaedics, Brown University, Providence, RI 02818, USA.
Acta Biomater. 2009 Jun;5(5):1425-32. doi: 10.1016/j.actbio.2009.01.031. Epub 2009 Feb 1.
Extensive prolonged interactions of inflammatory cells (such as macrophages) at the host-implant interface may lead to implant failure. While previous studies have shown increased in vitro and in vivo bone cell adhesion, proliferation and mineralization on nanophase compared to currently implanted ceramics, few studies have been conducted to elucidate inflammatory cell responses on such nanophase ceramics. Controlling surface feature size and corresponding surface roughness on implants may clearly alter immune cell responses, which would be an extremely important consideration for the use of nanostructured materials as improved biomaterials. In this study, reduced macrophage density was observed on alumina (Al(2)O(3)) compacts with greater nanometer surface roughness accompanied by changes in crystallinity for up to 24 h in culture. Since alumina is a commonly used ceramic in orthopedic applications, this in vitro study continues to support the use of nanophase ceramics as improved orthopedic implants by demonstrating reduced macrophage responses.
炎症细胞（如巨噬细胞）在宿主-植入物界面处广泛且长时间的相互作用可能导致植入物失败。虽然先前的研究表明，与目前植入的陶瓷相比，纳米相材料在体外和体内均能增强骨细胞的粘附、增殖和矿化，但很少有研究探讨纳米相陶瓷上炎症细胞的反应。控制植入物的表面特征尺寸和相应的表面粗糙度可能会明显改变免疫细胞反应，这对于将纳米结构材料用作改良生物材料来说是一个极其重要的考虑因素。在本研究中，在培养长达24小时的过程中，观察到纳米表面粗糙度更大且伴随着结晶度变化的氧化铝（Al(2)O(3)）压块上巨噬细胞密度降低。由于氧化铝是骨科应用中常用的陶瓷，这项体外研究通过证明巨噬细胞反应降低，继续支持将纳米相陶瓷用作改良的骨科植入物。
