Tingen Candace M, Bristol-Gould Sarah K, Kiesewetter Sarah E, Wellington Jason Tyler, Shea Lonnie, Woodruff Teresa K
Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Biol Reprod. 2009 Jul;81(1):16-25. doi: 10.1095/biolreprod.108.074898. Epub 2009 Mar 4.
More than half of the primordial follicles that are formed by Day 6 of postnatal life in the mouse will be eliminated from the ovary by the time of puberty. Apoptosis, a form of programmed cell death, is one mechanism by which these follicles could be actively lost. To investigate whether apoptosis is responsible for the loss of primordial follicles, follicular atresia was examined during the prepubertal period, when follicles die and are cleared from the ovary at an extremely high rate. Four hallmarks of classical apoptosis were measured in follicles present in prepubertal ovaries. The primordial follicle cohort was not positively associated with nuclear condensation or cell shrinkage, activation of caspase 3, cleavage of poly(ADP ribose) polymerase 1 (PARP1), or fragmentation of DNA. These data are consistent with a nonapoptotic pathway that is responsible for small follicle death.
在小鼠出生后第6天形成的原始卵泡中，超过一半在青春期时会从卵巢中被清除。凋亡是一种程序性细胞死亡形式，是这些卵泡可能被主动清除的一种机制。为了研究凋亡是否导致原始卵泡的丢失，在青春期前阶段对卵泡闭锁进行了检查，此时卵泡死亡并以极高的速率从卵巢中清除。对青春期前卵巢中存在的卵泡测量了经典凋亡的四个标志。原始卵泡群与核浓缩或细胞收缩、半胱天冬酶3的激活、聚（ADP核糖）聚合酶1（PARP1）的切割或DNA片段化均无正相关。这些数据与负责小卵泡死亡的非凋亡途径一致。
