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Nitroxyl activates SERCA in cardiac myocytes via glutathiolation of cysteine 674.
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Cysteine-674 of the sarco/endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide.
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HNO enhances SERCA2a activity and cardiomyocyte function by promoting redox-dependent phospholamban oligomerization.
Antioxid Redox Signal. 2013 Oct 10;19(11):1185-97. doi: 10.1089/ars.2012.5057.
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Oxidative posttranslational modifications mediate decreased SERCA activity and myocyte dysfunction in Galphaq-overexpressing mice.
Circ Res. 2010 Jul 23;107(2):228-32. doi: 10.1161/CIRCRESAHA.110.217570. Epub 2010 May 27.
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High glucose oxidizes SERCA cysteine-674 and prevents inhibition by nitric oxide of smooth muscle cell migration.
J Mol Cell Cardiol. 2008 Feb;44(2):361-9. doi: 10.1016/j.yjmcc.2007.10.022. Epub 2007 Nov 12.

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p22 prevents the oxidation of SERCA2a and stabilizes it in the heart.
Nat Cardiovasc Res. 2025 Sep 3. doi: 10.1038/s44161-025-00699-x.
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SERCA2a dysfunction in the pathophysiology of heart failure with preserved ejection fraction: a direct role is yet to be established.
Heart Fail Rev. 2025 May;30(3):545-564. doi: 10.1007/s10741-025-10487-1. Epub 2025 Jan 23.
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Disulfide stress and its role in cardiovascular diseases.
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Glutathione and Glutaredoxin-Key Players in Cellular Redox Homeostasis and Signaling.
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Editorial: The role of calcium and calcium binding proteins in cell physiology and disease.
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The interplay between cardiac dyads and mitochondria regulated the calcium handling in cardiomyocytes.
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ER stress and calcium-dependent arrhythmias.
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Skeletal and cardiac muscle calcium transport regulation in health and disease.
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2
Thiol oxidation in signaling and response to stress: detection and quantification of physiological and pathophysiological thiol modifications.
Free Radic Biol Med. 2007 Oct 15;43(8):1099-108. doi: 10.1016/j.freeradbiomed.2007.07.014. Epub 2007 Jul 19.
3
Cysteine-674 of the sarco/endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide.
Arterioscler Thromb Vasc Biol. 2007 Apr;27(4):783-90. doi: 10.1161/01.ATV.0000258413.72747.23. Epub 2007 Jan 18.
4
The pharmacology of nitroxyl (HNO) and its therapeutic potential: not just the Janus face of NO.
Pharmacol Ther. 2007 Feb;113(2):442-58. doi: 10.1016/j.pharmthera.2006.11.002. Epub 2006 Nov 29.
5
The nitroxyl anion (HNO) is a potent dilator of rat coronary vasculature.
Cardiovasc Res. 2007 Feb 1;73(3):587-96. doi: 10.1016/j.cardiores.2006.11.018. Epub 2006 Nov 18.
6
Nitroxyl improves cellular heart function by directly enhancing cardiac sarcoplasmic reticulum Ca2+ cycling.
Circ Res. 2007 Jan 5;100(1):96-104. doi: 10.1161/01.RES.0000253904.53601.c9. Epub 2006 Nov 30.
7
Peroxynitrite and myocardial contractility: in vivo versus in vitro effects.
Free Radic Biol Med. 2006 Nov 15;41(10):1606-18. doi: 10.1016/j.freeradbiomed.2006.08.023. Epub 2006 Sep 5.
8
S-glutathiolation of p21ras by peroxynitrite mediates endothelial insulin resistance caused by oxidized low-density lipoprotein.
Arterioscler Thromb Vasc Biol. 2006 Nov;26(11):2454-61. doi: 10.1161/01.ATV.0000242791.28953.4c. Epub 2006 Aug 24.
9
Strain-stimulated hypertrophy in cardiac myocytes is mediated by reactive oxygen species-dependent Ras S-glutathiolation.
J Mol Cell Cardiol. 2006 Oct;41(4):613-22. doi: 10.1016/j.yjmcc.2006.05.009. Epub 2006 Jun 27.
10
Inhibition of yeast glycolysis by nitroxyl (HNO): mechanism of HNO toxicity and implications to HNO biology.
Arch Biochem Biophys. 2005 Oct 1;442(1):140-8. doi: 10.1016/j.abb.2005.07.012.

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