Kurtti T J, Brooks M A
In Vitro. 1977 Jan;13(1):11-7. doi: 10.1007/BF02615498.
Cell lines were isolated from three stages of embryos of Blattella germanica dissociated with trypsin. The lines have been subcultured 50 to 134 times in 3 years. Line UM-BGE-1 was isolated from germ band embryos at stages of segmentation and limb-bud formation (5 days old). Line UM-BGE-2 was derived from embryos at dorsal closure (7 days old). Line UM-BGE-4 arose from embryos in the germ band and dorsal closure stages (5 and 7 days old): these cells colonize as hollow spheres or vesicles. Line UM-BGE-5, isolated during organogenesis (10 days old), developed into two distinct sublines. Subline alpha is composed of round cells that do not attach to the flask. Subline beta grows as an attached monolayer; the cells can be removed with a saline solution containing 20 mM disodium dihydrogen Versenate. Most of the cells of these lines have the diploid chromosome number (23 or 24) excepting line UM-BGE-1 in which the tetraploid number predominates
细胞系从经胰蛋白酶解离的德国小蠊胚胎的三个阶段分离得到。这些细胞系在3年中传代培养了50至134次。UM-BGE-1细胞系从处于分节和肢芽形成阶段（5日龄）的胚带胚胎中分离得到。UM-BGE-2细胞系源自背闭合阶段（7日龄）的胚胎。UM-BGE-4细胞系来自胚带和背闭合阶段（5日龄和7日龄）的胚胎：这些细胞以空心球体或囊泡的形式定植。UM-BGE-5细胞系在器官发生期（10日龄）分离得到，发展成两个不同的亚系。α亚系由不附着于培养瓶的圆形细胞组成。β亚系以贴壁单层的形式生长；细胞可用含20 mM乙二胺四乙酸二钠的盐溶液去除。除了以四倍体数为主的UM-BGE-1细胞系外,这些细胞系的大多数细胞具有二倍体染色体数（23或24条）
