Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China.
Ophthalmic Res. 2013;49(3):150-60. doi: 10.1159/000343775. Epub 2012 Dec 18.
This study was undertaken to investigate the effects of recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (bFGF) on corneal wound healing and neovascularization (CNV).
The positive effects of 10 ng/ml rhEGF and bFGF on the proliferation of corneal epithelial cells (SD-HCEC1s), rabbit keratocyte cells (RKCs) and human umbilical vein endothelial cells (HUVECs) as well as the effects on the migration capacity on HUVECs were observed. An animal central corneal wound and CNV model was established in rabbits. One eye of each group was chosen randomly for topical administration of rhEGF, bFGF or normal saline, and variability in the area of corneal epithelial wound healing and CNV was observed.
The optimal concentration of rhEGF and bFGF for the proliferation of corneal epithelial cells was 10 ng/ml. The promotive effect of 10 ng/ml rhEGF on the proliferation of RKCs and HUVECs was less than that of 10 ng/ml bFGF. In the animal experiment, the healing rate of the corneal epithelium in the rhEGF group was better than in the other groups on day 1. On day 3, the healing rates of the 3 groups were nearly equal. The CNV area in the rhEGF group was less than that of the bFGF group.
rhEGF and bFGF both had promotive effects on corneal epithelial wound healing, but rhEGF had a weaker promotive effect on CNV than bFGF. With long-term application of growth factor drugs, rhEGF is suggested for lessening the growth of CNV.
本研究旨在探讨重组人表皮生长因子（rhEGF）和碱性成纤维细胞生长因子（bFGF）对角膜伤口愈合和新生血管形成（CNV）的影响。
观察 10ng/ml rhEGF 和 bFGF 对角膜上皮细胞（SD-HCEC1s）、兔角膜基质细胞（RKCs）和人脐静脉内皮细胞（HUVECs）增殖的促进作用以及对 HUVECs 迁移能力的影响。建立兔中央角膜伤口和 CNV 模型。随机选择每组一只眼进行 rhEGF、bFGF 或生理盐水局部给药，观察角膜上皮伤口愈合和 CNV 面积的变化。
rhEGF 和 bFGF 促进角膜上皮细胞增殖的最佳浓度均为 10ng/ml。10ng/ml rhEGF 对 RKCs 和 HUVECs 增殖的促进作用小于 10ng/ml bFGF。在动物实验中，rhEGF 组角膜上皮愈合率在第 1 天优于其他组。第 3 天，3 组的愈合率几乎相等。rhEGF 组的 CNV 面积小于 bFGF 组。
rhEGF 和 bFGF 均对角膜上皮伤口愈合有促进作用，但 rhEGF 对 CNV 的促进作用弱于 bFGF。长期应用生长因子药物时，建议使用 rhEGF 以减少 CNV 的生长。
