Moody Richard P, Joncas Julie, Richardson Mark, Petrovic Sanya, Chu Ih
Exposure and Bio-Monitoring Division, Dermal Absorption Lab Unit, Health Canada, Ottawa ONK1AOK9, Canada.
J Toxicol Environ Health A. 2009;72(8):551-9. doi: 10.1080/15287390802706322.
Dermal absorption of heavy metal soil contaminants was tested in vitro with chloride salts of radioactive nickel (Ni-63) and mercury (Hg-203). Aqueous soil suspensions, spiked with either Ni-63 or Hg-203, were applied to fresh viable human breast skin tissue in Bronaugh diffusion cells perfused with Hanks HEPES buffered (pH 7.4) receptor containing 4% bovine serum albumin (BSA). Receptor fractions were collected every 6 h for 24 h when skin was soap washed. Tests were conducted concurrently in triplicate with and without soil for each skin specimen. Mean percent dermal absorption including the skin depot for Ni-63 was 1 and 22.8% with and without soil, respectively, while for Hg-203, values of 46.6 and 78.3% were obtained. Excluding the skin depot and considering only absorption in receptor, there was 0.5 and 1.8% absorption of Ni-63 with and without soil, respectively, and 1.5 and 1.4% for Hg-203. The potential bioavailability of the skin depot is discussed in relation to dermal exposure to these metals in contaminated soil.
采用放射性镍（Ni - 63）和汞（Hg - 203）的氯化物盐，在体外测试了重金属土壤污染物的皮肤吸收情况。将添加了Ni - 63或Hg - 203的土壤水悬浮液，应用于布罗瑙扩散池中新鲜有活力的人乳房皮肤组织，该扩散池灌注了含4%牛血清白蛋白（BSA）的汉克斯 - HEPES缓冲液（pH 7.4）受体溶液。当皮肤用肥皂清洗时，每6小时收集一次受体部分，持续24小时。对每个皮肤样本，分别在有土壤和无土壤的情况下同时进行三次重复试验。包括皮肤贮库在内，Ni - 63有土壤和无土壤时的平均皮肤吸收百分比分别为1%和22.8%，而Hg - 203的相应值分别为46.6%和78.3%。不考虑皮肤贮库，仅考虑受体中的吸收情况，有土壤和无土壤时Ni - 63的吸收分别为0.5%和1.8%，Hg - 203的吸收分别为1.5%和1.4%。结合在受污染土壤中皮肤接触这些金属的情况，讨论了皮肤贮库的潜在生物有效性。
