Exposure and Bio-Monitoring Division, Dermal Absorption Lab Unit, Health Canada, Ottawa, Ontario.
J Toxicol Environ Health A. 2010;73(15):1021-31. doi: 10.1080/15287394.2010.481617.
Dermal absorption of contaminants from soils at federal contaminated sites in Canada was investigated using one hydrophile, (14)C-ethylene glycol (EG), and one lipophile, (14)C-nonylphenol (NP). In vitro dermal absorption of EG and NP was examined in dermatomed (0.4-0.5 mm) human skin using Bronaugh Teflon flow-through cells with Hanks HEPES buffered (pH 7.4) receiver solution with 4% bovine serum albumin (BSA). Tests were conducted under occlusive conditions with and without a commercial gardening soil spiked with EG or NP applied to skin at a soil load of 5 mg/cm(2). With percent absorption in skin depot included, a total of 9.9 + or - 6.28% (n = 6) and 34.8 + or - 8.47% (n = 6) absorption of EG with and without soil, respectively, and 20.6 + or - 5.56% (n = 7) and 41.1 + or - 6.46% (n = 7) of NP, with and without soil, respectively, were obtained. For tests without soil a reverse pattern was observed with significantly lower percent absorption into the receiver than depot with the lipophile NP, but significantly higher percent absorption in receiver versus depot for the hydrophile EG. This pattern was different in tests with soil, and caution needs to be exercised when extrapolating data from in vitro tests conducted without soil in human health risk assessments at contaminated sites.
加拿大联邦污染场地土壤中污染物的经皮吸收情况研究，选用亲水性物质（14）C-乙二醇（EG）和疏水性物质（14）C-壬基酚（NP）进行研究。采用 Bronaugh 特氟隆流动细胞，以 Hank's HEPES 缓冲（pH7.4）含 4%牛血清白蛋白（BSA）的接收液，对去皮（0.4-0.5mm）的人体皮肤进行体外 EG 和 NP 经皮吸收试验。试验在密闭条件下进行，有和无商业化园艺土（添加 EG 或 NP）施用于皮肤，土壤负荷为 5mg/cm(2)。将皮肤库中的吸收百分比包括在内，分别有 9.9+/-6.28%（n=6）和 34.8+/-8.47%（n=6）的 EG 有和无土壤吸收，以及 20.6+/-5.56%（n=7）和 41.1+/-6.46%（n=7）的 NP 有和无土壤吸收。对于没有土壤的试验，亲脂性 NP 进入接收液的吸收百分比明显低于库，而疏水性 EG 则相反，进入接收液的吸收百分比明显高于库。这种模式在有土壤的试验中有所不同，在对污染场地人体健康风险评估中，需要谨慎地将无土壤体外试验数据外推。
