Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Shanda Nanlu 27#, Jinan 250100, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Feb;78(2):681-6. doi: 10.1016/j.saa.2010.11.048. Epub 2010 Dec 7.
A novel method for the determination of nucleic acids by using silver nanoparticle (AgNPs)-eriochrome black T (EBT) as a resonance light scattering (RLS) probe has been developed. Under optimum conditions, there are linear relationships between the quenching extent of RLS intensity and the concentration of nucleic acids in the range of 4.0×10(-9)-4.0×10(-7), 4.0×10(-7)-1.6×10(-6) g mL(-1) for fish sperm DNA (fsDNA) and 4.0×10(-8)-2.0×10(-6) g mL(-1) for yeast RNA (yRNA). Their detection limits (S/N=3) are 2.0 ng mL(-1) and 21 ng mL(-1), respectively. The results indicate that AgNPs can form wirelike aggregates and nanoslices in the presence of the EBT. Whereas, when nucleic acids are added into the AgNPs-EBT system, the dynamic balance of AgNPs-EBT system is destroyed and the nanoparticles undergo dispersion again, leading to the RLS intensity of AgNPs-EBT system quenching. Meanwhile, the conformation of fsDNA is changed by the synergistic effect of AgNPs and EBT.
一种利用银纳米粒子（AgNPs）-铬黑 T（EBT）作为共振光散射（RLS）探针测定核酸的新方法已经建立。在最佳条件下，RLS 强度的猝灭程度与核酸的浓度在 4.0×10(-9)-4.0×10(-7)、4.0×10(-7)-1.6×10(-6) g mL(-1)范围内呈线性关系，用于鱼精 DNA（fsDNA），在 4.0×10(-8)-2.0×10(-6) g mL(-1)范围内用于酵母 RNA（yRNA）。它们的检测限（S/N=3）分别为 2.0 ng mL(-1)和 21 ng mL(-1)。结果表明，在 EBT 的存在下，AgNPs 可以形成线状聚集体和纳米薄片。然而，当核酸被加入到 AgNPs-EBT 体系中时，AgNPs-EBT 体系的动态平衡被破坏，纳米粒子再次分散，导致 AgNPs-EBT 体系的 RLS 强度猝灭。同时，fsDNA 的构象通过 AgNPs 和 EBT 的协同作用发生变化。
