Zhao Ran, Li Geng, Kong Xiu-Juan, Huang Xiu-Yan, Li Wei, Zeng Yao-Ying, Lai Xiao-Ping
Traditional Chinese Medicinal College, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
Life Science College, Jinan University, Guangzhou, People's Republic of China.
Drug Des Devel Ther. 2016 Jan 21;10:371-81. doi: 10.2147/DDDT.S88193. eCollection 2016.
Edible bird's nest (EBN) is regarded as an immune-enhancing food in the People's Republic of China. The aim of this study is to demonstrate the efficiency of EBN in improving the immunity of mouse both in vivo and in vitro. We observed the effects of EBN on spleen lymphocytes proliferation and activation, as well as immunoglobulin isotypes as indicators. In addition, we evaluated the content of total sIgA in the intestinal juice to assess mucosal immunity. The results showed that EBN could promote the proliferation and activation of B-cells and increase IgE, IgA, IgM, and IgG3 levels. We also found that EBN extract can promote the secretion of sIgA in the small intestine. Using cyclophosphamide (CY), we established an immunosuppressed mouse model in which we identified a reversal influence on the ratio of CD3(+)/CD19(+) cells, which indicates that EBN also protects B-cells from the damage induced by CY. We also applied polymyxin B to exclude the interference of lipopolysaccharide throughout the experiment. In conclusion, we found that EBN can reduce the intestinal immune injury induced by CY by accelerating the proliferation and activation of B-cells and enhancing antibody secretion of B-cells.
在中国，食用燕窝被视为一种增强免疫力的食物。本研究的目的是证明食用燕窝在体内和体外提高小鼠免疫力方面的功效。我们以燕窝对脾淋巴细胞增殖和活化的影响以及免疫球蛋白同种型作为指标进行观察。此外，我们评估了肠液中总分泌型免疫球蛋白A（sIgA）的含量以评估黏膜免疫。结果表明，燕窝可促进B细胞的增殖和活化，并提高免疫球蛋白E（IgE）、免疫球蛋白A（IgA）、免疫球蛋白M（IgM）和免疫球蛋白G3（IgG3）的水平。我们还发现燕窝提取物可促进小肠中sIgA的分泌。我们使用环磷酰胺（CY）建立了免疫抑制小鼠模型，在此模型中我们确定了燕窝对CD3(+)/CD19(+)细胞比例具有逆转影响，这表明燕窝还能保护B细胞免受CY诱导的损伤。我们在整个实验过程中还应用多粘菌素B以排除脂多糖的干扰。总之，我们发现燕窝可通过加速B细胞的增殖和活化以及增强B细胞的抗体分泌来减轻CY诱导的肠道免疫损伤。
