Benzonana G, Wnuk W, Cox J A, Gabbiani G
Histochemistry. 1977 Apr 4;51(4):335-41. doi: 10.1007/BF00494370.
Specific antibodies against carp paravalbumin, crayfish calcium binding protein and crayfish arginine kinase were used for indirect immunofluorescence localization of the respective proteins. Simultaneous staining of the same muscle sections with human serum containing anti-actin autoantibodies served as a probe to identify the isotropic band. Parvalbumin appears to be evenly distributed in carp white muscle. The crayfish calcium binding protein however shows a distinct localization, in the isotropic band, coincident with the actin staining. Arginine kinase, which has the same molecular weight and is extractible in the same way as the calcium binding protein does not show this distinct localization, but is evenly present in crayfish tail muscle, similarly to parvalbumin. The possible meaning of the different distribution of the two calcium binding proteins is discussed.
针对鲤鱼副肌球蛋白、小龙虾钙结合蛋白和小龙虾精氨酸激酶的特异性抗体被用于各自蛋白质的间接免疫荧光定位。用含有抗肌动蛋白自身抗体的人血清对相同的肌肉切片进行同步染色，作为识别各向同性带的探针。副肌球蛋白似乎均匀分布在鲤鱼白肌中。然而，小龙虾钙结合蛋白显示出明显的定位，在各向同性带中，与肌动蛋白染色一致。精氨酸激酶与钙结合蛋白具有相同的分子量且提取方式相同，但并未显示出这种明显的定位，而是像副肌球蛋白一样均匀存在于小龙虾尾肌中。讨论了两种钙结合蛋白不同分布的可能意义。
