Ishikawa Tomomi, Nanjo Fumio
Food Research Laboratories, Mitsui Norin Co., Ltd., Fujieda, Shizuoka, Japan.
Biosci Biotechnol Biochem. 2009 Mar 23;73(3):677-82. doi: 10.1271/bbb.80733. Epub 2009 Mar 7.
The purpose of this study was to elucidate the effects of dietary supplemented cycloinulooligosaccharides (CF) on the intestinal immune function and humoral immunity in BALB/c mice. The mice were orally administered with a control diet or a diet containing 5% CF for 6 weeks. The fecal IgA level, an indicator of the intestinal immune response, was increased dramatically by 5% CF feeding, and casein-specific IgA in the feces was also significantly increased. The amounts of short-chain fatty acids (SCFA) in the cecal contents and feces were significantly higher in the 5% CF group than in the control group. On the other hand, CF administration had only a slight affect on the plasma IgA and IgG levels and no effect on the plasma IgE and IgM levels. These results indicate that dietary CF up-regulated the intestinal immune response, but not the humoral immune response. Furthermore, the dose-dependent effect of CF feeding on IgA and SCFA production was examined. Significant increases in the fecal IgA and SCFA levels were observed in the mice fed with 5% CF, but not in the 1% and 2.5% CF-fed groups, indicating that the amount of CF administered was an important factor for up-regulation of the intestinal immune function.
本研究的目的是阐明日粮添加环低聚果糖（CF）对BALB/c小鼠肠道免疫功能和体液免疫的影响。将小鼠分别口服对照日粮或含5%CF的日粮，持续6周。作为肠道免疫反应指标的粪便IgA水平，在饲喂5%CF后显著升高，粪便中酪蛋白特异性IgA也显著增加。5%CF组盲肠内容物和粪便中的短链脂肪酸（SCFA）含量显著高于对照组。另一方面，给予CF对血浆IgA和IgG水平仅有轻微影响，对血浆IgE和IgM水平无影响。这些结果表明，日粮CF上调了肠道免疫反应，但未上调体液免疫反应。此外，还研究了CF饲喂对IgA和SCFA产生的剂量依赖性效应。在饲喂5%CF的小鼠中观察到粪便IgA和SCFA水平显著增加，但在饲喂1%和2.5%CF的组中未观察到，这表明给予CF的量是上调肠道免疫功能的重要因素。
