Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonju, Jeonbuk 561-756 Chonbuk National University, Jeonju, Jeonbuk, Korea.
Vector Borne Zoonotic Dis. 2009 Dec;9(6):681-6. doi: 10.1089/vbz.2008.0116.
Clinically diagnosed cases of Babesia gibsoni infection in dogs were evaluated on the basis of polymerase chain reaction (PCR) results and nucleotide sequences of the 18S rRNA gene. Twenty-nine of 117 submitted dogs were PCR positive. The breed of dogs infected with B. gibsoni varied; however, pit bull terriers were the breed most often infected. The infection rate was higher in densely populated regions and in male dogs. Young dogs (age < 3 years) were more sensitive to B. gibsoni infection, and 6 out of the 29 infected dogs had a history of tick exposure. The clinical signs observed during physical examination were related to canine babesiosis, and many dogs showed symptoms similar to those associated with anemia. The results of hematologic analysis revealed severe hemolytic anemia and thrombocytopenia in the infected dogs. However, the blood smears of 29 infected dogs showed very low levels of parasitemia. Nucleotide sequencing of the PCR products revealed that the 18S rRNA gene sequences of B. gibsoni in South Korea were very close to those reported in Spain, Japan, and the United States.
基于聚合酶链反应（PCR）结果和 18S rRNA 基因的核苷酸序列，对临床诊断的感染巴贝斯虫病的犬只进行了评估。在提交的 117 只犬中，有 29 只是 PCR 阳性。感染巴贝斯虫的犬种各不相同；然而，比特犬是最常感染的犬种。在人口密集地区和雄性犬中，感染率较高。幼犬（<3 岁）对巴贝斯虫感染更为敏感，在 29 只感染犬中，有 6 只有蜱暴露史。体格检查中观察到的临床症状与犬巴贝斯虫病有关，许多犬表现出与贫血相关的类似症状。感染犬的血液学分析结果显示严重的溶血性贫血和血小板减少症。然而，29 只感染犬的血涂片显示极低水平的寄生虫血症。PCR 产物的核苷酸序列分析显示，韩国的巴贝斯虫 18S rRNA 基因序列与西班牙、日本和美国报道的序列非常接近。
