Walpusk Janet, Galambos Csaba
Department of Pediatric Pathology, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, USA.
Ultrastruct Pathol. 2009 Mar-Apr;33(2):76-82. doi: 10.1080/01913120902751361.
Despite the widespread utilization of immunohistochemical stains in pathologic practice, virus identification remains a challenge. In our institution, electron microscopy combined with the lift technique has been utilized for 40 years as a reliable diagnostic tool where a question of viral infection is raised by light microscopic observation and could not be otherwise confirmed. The combination of light microscopic and ultrastructural methods has allowed us to examine individual cells suspicious for harboring viral particles previously identified on a hematoxylin-eosin stained tissue section. In this review we describe the lift technique in detail with our modifications in the hope that the use of lift technique in these uncommon but specific circumstances will prove clinically valuable.
尽管免疫组织化学染色在病理实践中得到广泛应用，但病毒鉴定仍然是一项挑战。在我们机构，电子显微镜结合提升技术作为一种可靠的诊断工具已使用了40年，用于解决光镜观察提出病毒感染问题但无法通过其他方式确诊的情况。光镜和超微结构方法的结合使我们能够检查在苏木精-伊红染色组织切片上先前鉴定出的疑似含有病毒颗粒的单个细胞。在本综述中，我们详细描述了提升技术及其改进，希望在这些罕见但特定的情况下使用提升技术将证明具有临床价值。
