Smith J, Coleman D V
Acta Cytol. 1983 Nov-Dec;27(6):605-13.
A technique is described whereby cells showing cytologic changes suggestive of virus infection by light microscopy can be processed further for examination in the electron microscope so that virus particles present in the cell can be visualized directly. We present the results of electron microscopy of over 100 Papanicolaou-stained smears processed this way. The morphologic changes in the cells and the ultrastructural appearances of the virus particles are demonstrated. This technique is particularly valuable for retrospective studies of mounted cytologic or histologic material. It has also proven to be a valuable research tool in the study of human polyomavirus and human wart virus infection.
本文描述了一种技术，通过该技术，在光学显微镜下显示出提示病毒感染的细胞学变化的细胞可以进一步处理，以便在电子显微镜下进行检查，从而可以直接观察到细胞中存在的病毒颗粒。我们展示了用这种方法处理的100多张巴氏染色涂片的电子显微镜检查结果。文中展示了细胞的形态学变化以及病毒颗粒的超微结构外观。这项技术对于已制备好的细胞学或组织学材料的回顾性研究特别有价值。它也已被证明是研究人类多瘤病毒和人类乳头瘤病毒感染的一种有价值的研究工具。
