Department of Medical Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Plateau State College of Health Technology, Zawan, Jos, Nigeria.
PLoS One. 2022 May 24;17(5):e0266837. doi: 10.1371/journal.pone.0266837. eCollection 2022.
Nigeria ranks 1st in Africa and 6th globally with the highest burden of tuberculosis (TB). However, only a relatively few studies have addressed the molecular epidemiology of Mycobacterium tuberculosis in this country. The aim of this work was to analyze the genetic structure of drug-resistant (DR) M. tuberculosis population in the Plateau State (central Nigeria), with the results placed in the broader context of West Africa. The study sample included 67 DR M. tuberculosis isolates, recovered from as many TB patients between November 2015 and January 2016, in the Plateau State. The isolates were subjected to spoligotyping and MIRU-VNTR typing. A total of 20 distinct spoligotypes were obtained, split into 3 clusters (n = 50, 74.6%, 2-33 isolates per cluster) and 17 (25.4%) unique patterns. The Cameroon clade was the largest lineage (62.7%) followed by T (28.3%), LAM (3%), and Haarlem (3%) clades. Upon MIRU-VNTR typing, the isolates produced 31 profiles, i.e. 7 clusters (n = 43, 64.2%, 2-17 isolates per cluster) and 24 singletons. A combined spoligotyping and MIRU-VNTR typing analysis showed 20.9% of the cases clustered and estimated the recent transmission rate at 11.9%. In conclusion, two lineages, namely Cameroon, and T accounted for the majority (91%) of cases. No association was observed between the most prevalent Cameroon lineage and drug resistance, including multidrug resistant (MDR) phenotype, or any of the patient demographic characteristics.
尼日利亚在非洲排名第一，在全球排名第六，结核病（TB）负担最重。然而，只有相对较少的研究涉及该国结核分枝杆菌的分子流行病学。这项工作的目的是分析尼日利亚高原州（中部）耐药（DR）结核分枝杆菌人群的遗传结构，并将结果置于西非的更广泛背景下。研究样本包括 2015 年 11 月至 2016 年 1 月期间从高原州的 67 名结核病患者中分离出的 67 株 DR 结核分枝杆菌。对分离株进行 spoligotyping 和 MIRU-VNTR 分型。共获得 20 种不同的 spoligotype，分为 3 个簇（n = 50，74.6%，每个簇 2-33 个分离株）和 17 个（25.4%）独特模式。喀麦隆分支是最大的谱系（62.7%），其次是 T（28.3%）、LAM（3%）和 Haarlem（3%）分支。通过 MIRU-VNTR 分型，分离株产生 31 种谱型，即 7 个簇（n = 43，64.2%，每个簇 2-17 个分离株）和 24 个单倍型。 spoligotyping 和 MIRU-VNTR 联合分型分析显示，20.9%的病例聚类，并估计最近的传播率为 11.9%。总之，两个谱系，即喀麦隆和 T，占大多数（91%）的病例。最常见的喀麦隆谱系与耐药性，包括耐多药（MDR）表型或任何患者人口统计学特征之间没有关联。
