Pan Hung-Chuan, Wu Hsi-Tien, Cheng Fu-Chou, Chen Cheng-Hsu, Sheu Meei-Ling, Chen Chun-Jung
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 407, Taiwan.
Biochem Biophys Res Commun. 2009 Apr 24;382(1):177-82. doi: 10.1016/j.bbrc.2009.03.003. Epub 2009 Mar 9.
Granulocyte colony-stimulating factor (G-CSF) demonstrates neuroprotective effects through different mechanisms, including mobilization of bone marrow cells. However, the influence of G-CSF-mediated mobilization of bone marrow-derived cells on injured sciatic nerves remains to be elucidated. The administration of G-CSF promoted a short-term functional recovery 7 days after crush injury in sciatic nerves. A double-immunofluorescence study using green fluorescent protein-chimeric mice revealed that bone marrow-derived CD34+ cells were predominantly mobilized and migrated into injured nerves after G-CSF treatment. G-CSF-mediated beneficial effects against sciatic nerve injury were associated with increased CD34+ cell deposition, vascular endothelial growth factor (VEGF) expression, and vascularization/angiogenesis as well as decreased CD68+ cell accumulation. However, cell differentiation and VEGF expression were not demonstrated in deposited cells. The results suggest that the promotion of short-term functional recovery in sciatic nerve crush injury by G-CSF involves a paracrine modulatory effect and a bone marrow-derived CD34+ cell mobilizing effect.
粒细胞集落刺激因子（G-CSF）通过不同机制发挥神经保护作用，包括动员骨髓细胞。然而，G-CSF介导的骨髓来源细胞动员对坐骨神经损伤的影响仍有待阐明。G-CSF给药促进了坐骨神经挤压伤后7天的短期功能恢复。使用绿色荧光蛋白嵌合小鼠的双免疫荧光研究表明，G-CSF治疗后，骨髓来源的CD34+细胞主要被动员并迁移到受损神经中。G-CSF介导的对坐骨神经损伤的有益作用与CD34+细胞沉积增加、血管内皮生长因子（VEGF）表达、血管生成/血管新生增加以及CD68+细胞积累减少有关。然而，在沉积细胞中未观察到细胞分化和VEGF表达。结果表明，G-CSF促进坐骨神经挤压伤短期功能恢复涉及旁分泌调节作用和骨髓来源的CD34+细胞动员作用。
