Yu Joo-In, Cho Yeong-Hyun, Seo Tae-Beom, Kim Young-Pyo
Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea.
J Exerc Rehabil. 2023 Feb 23;19(1):19-26. doi: 10.12965/jer.2244006.003. eCollection 2023 Feb.
The purpose of this study was to determine whether combined inter-vention of treadmill exercise and bone marrow stromal cell (BMSC) transplantation would affect the expression of neurotrophic factors in the sciatic nerve injury (SNI) and neuropathic pain-related cascades in ipsilateral lumbar 4-5 dorsal root ganglion (DRG) during the early or late stage of sciatic nerve regeneration. The rats were randomly divided into the normal control group (CONT, n=6), sedentary group (SS, n=24), exercise group (SE, n=24), BMSC transplantation group (SB, n=24), BMSC transplantation+exercise group (SBE, n=24) 1, 2, 3, and 5 weeks after SNI. Single dose of 5×10 harvested BMSC was injected into the injury area sing by a 30 gauge needle. Treadmill exercise was performed at a speed of 8 m/min for 30 min once a day. Tropomyosin-receptor kinase B, brain-derived neurotrophic factor and ciliary neurotrophic fac-tor were significantly upregulated in the SE and SBE groups at 1- and 2-week postinjury than those in the CONT and SS groups, and SB and SBE groups continuously kept up proinflammatory cytokines until the late stage of regeneration. Nuclear factor kappa-light-chain-enhancer of activated B cells, interleukin and tumor necrosis factor alpha in ipsi-lateral DRG were progressively decreased by exercise alone application and/or BMSC transplantation at early and late stage of regeneration. Present results provide reliable information that combined intervention of treadmill exercise and BMSC transplantation might be one of the effective treatment strategies for recovering sciatic nerve injury-induced neuropathic pain over time.
本研究的目的是确定跑步机运动和骨髓基质细胞（BMSC）移植的联合干预是否会影响坐骨神经再生早期或晚期坐骨神经损伤（SNI）中神经营养因子的表达以及同侧腰4-5背根神经节（DRG）中与神经性疼痛相关的级联反应。大鼠在SNI后1、2、3和5周被随机分为正常对照组（CONT，n = 6）、久坐组（SS，n = 24）、运动组（SE，n = 24）、BMSC移植组（SB，n = 24）、BMSC移植+运动组（SBE，n = 24）。通过30号针头将单剂量5×10收获的BMSC注射到损伤区域。跑步机运动以8米/分钟的速度每天进行一次，每次30分钟。与CONT组和SS组相比，SE组和SBE组在损伤后1周和2周时原肌球蛋白受体激酶B、脑源性神经营养因子和睫状神经营养因子显著上调，并且SB组和SBE组在再生后期持续保持促炎细胞因子水平。在再生的早期和晚期，单独运动和/或BMSC移植可使同侧DRG中的活化B细胞核因子κB轻链增强子、白细胞介素和肿瘤坏死因子α逐渐降低。目前的结果提供了可靠的信息，即跑步机运动和BMSC移植的联合干预可能是随着时间推移恢复坐骨神经损伤所致神经性疼痛的有效治疗策略之一。
