Zhang X, Stamps W T, Linit M J
J Nematol. 1995 Mar;27(1):36-41.
Pine wilt is caused by the nematode Bursaphelenchus xylophilus, which is transported to host trees in the trachea of Monochamus spp. (Coleoptera: Cerambycidae). The study of the relationship between the nematode and its beetle vectors has been hampered by the inability to estimate nematode presence or density within live beetles. This report describes a rapid method for estimating nematode load within live M. carolinensis and M. alternatus by visual examination of the atrium of the first abdominal spiracle. Visual estimates of nematode numbers correlated highly with actual nematode numbers. This method is a timesaving technique for determining relative numbers of B. xylophilus in pine wilt research.
松材线虫病由线虫松材线虫（Bursaphelenchus xylophilus）引起，该线虫通过松墨天牛属（鞘翅目：天牛科）昆虫的气管被传播到寄主树木上。由于无法估计活虫体内线虫的存在情况或密度，线虫与其甲虫传播媒介之间关系的研究受到了阻碍。本报告描述了一种通过目视检查第一腹节气门心房来快速估计活的卡罗来纳松墨天牛和松墨天牛体内线虫负荷的方法。线虫数量的目视估计与实际线虫数量高度相关。该方法是在松材线虫病研究中确定松材线虫相对数量的一种省时技术。
