Zhang Zi-Yi, Guan Jia-Yin, Cao Yu-Rou, Dai Xin-Yi, Storey Kenneth B, Yu Dan-Na, Zhang Jia-Yong
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
Insects. 2021 May 14;12(5):453. doi: 10.3390/insects12050453.
We determined the mitochondrial gene sequence of and three other mitogenomes of Lamiinae (Insect: Coleoptera: Cerambycidae) belonging to three genera (, and ) to enrich the mitochondrial genome database of Lamiinae and further explore the phylogenetic relationships within the subfamily. Phylogenetic trees of the Lamiinae were built using the Bayesian inference (BI) and maximum likelihood (ML) methods and the monophyly of , , and genera was supported. , and were closely related, suggesting they may also be potential vectors for the transmission of the pine wood pathogenic nematode () in addition to , a well-known vector of pine wilt disease. There is a special symbiotic relationship between and . As the native sympatric sibling species of , also has a specific relationship that is often overlooked. The analysis of mitochondrial gene expression aimed to explore the effect of on the energy metabolism of the respiratory chain of adults. Using RT-qPCR, we determined and analyzed the expression of eight mitochondrial protein-coding genes (, , , , , , , and ) between infected by and without the nematode. Expression of all the eight mitochondrial genes were up-regulated, particularly the and gene, which were up-regulated by 4-5-fold ( < 0.01). Since longicorn beetles have immune responses to nematodes, we believe that their relationship should not be viewed as symbiotic, but classed as parasitic.
我们测定了[某一物种名称]以及天牛亚科（昆虫纲：鞘翅目：天牛科）其他三个线粒体基因组的线粒体基因序列，这三个线粒体基因组分别属于三个属（[三个属的名称]），目的是丰富天牛亚科的线粒体基因组数据库，并进一步探究该亚科内的系统发育关系。利用贝叶斯推断（BI）和最大似然法（ML）构建了天牛亚科的系统发育树，[三个属的名称]属的单系性得到了支持。[三个属的名称]关系密切，这表明除了松材线虫病的著名传播媒介[某一物种名称]外，它们可能也是松材致病线虫（[线虫名称]）传播的潜在媒介。[两个物种名称]之间存在特殊的共生关系。作为[某一物种名称]的本地同域近缘物种，[另一物种名称]也有一段常被忽视的特殊关系。线粒体基因表达分析旨在探究[某一物种名称]对[另一物种名称]成虫呼吸链能量代谢的影响。我们使用逆转录定量聚合酶链反应（RT-qPCR）测定并分析了受[线虫名称]感染的[另一物种名称]和未受线虫感染的[另一物种名称]之间八个线粒体蛋白质编码基因（[八个基因名称]）的表达。所有八个线粒体基因的表达均上调，尤其是[两个基因名称]基因，上调了4至5倍（P<0.01）。由于天牛对线虫有免疫反应，我们认为它们之间的关系不应被视为共生，而应归类为寄生。
