用于寡糖结构解析的红外多光子解离质谱法。

Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides.

作者信息

Li Bensheng, An Hyun Joo, Hedrick Jerry L, Lebrilla Carlito B

机构信息

Department of Chemistry, University of California, Davis, CA, USA.

出版信息

Methods Mol Biol. 2009;534:23-35. doi: 10.1007/978-1-59745-022-5_2.

DOI:10.1007/978-1-59745-022-5_2

PMID:19277545

Abstract

The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation on the basis of tandem mass spectrometry. Ions are commonly selected and subjected to collision-induced dissociation (CID) to obtain structural information. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In this chapter, we illustrate the use of infrared multiphoton dissociation (IRMPD) to obtain structural information for O- and N-linked oligosaccharides. The IRMPD and CID behaviors of oligosaccharides are compared.

摘要

寡糖的结构解析仍然是一项重大挑战。质谱法基于串联质谱提供了一种快速便捷的结构解析方法。通常选择离子并使其经受碰撞诱导解离（CID）以获得结构信息。然而，CID的一个缺点是随着质量增加，解离程度和效率都会降低。在本章中，我们阐述了使用红外多光子解离（IRMPD）来获取O-连接和N-连接寡糖的结构信息。并比较了寡糖的IRMPD和CID行为。