Strobel Ingmar, Breitenbach Jürgen, Scheckhuber Christian Q, Osiewacz Heinz D, Sandmann Gerhard
Department of Biological Sciences and Cluster of Excellence Macromolecular Complexes, Institute of Molecular Biosciences, J. W. Goethe University, Frankfurt, Germany.
Curr Genet. 2009 Apr;55(2):175-84. doi: 10.1007/s00294-009-0235-0. Epub 2009 Mar 10.
Carotenoids have been identified in the fungus Podospora anserina and a parallel pathway to neurosporene and beta-carotene was established. Three genes for the beta-carotene branch have been cloned and their function elucidated. They correspond to the al-1, al-2 and al-3 genes from Neurospora crassa. They were individually and in combinations over-expressed in P. anserina in order to modify the carotenoid composition qualitatively and quantitatively. In the resulting transformants, carotenoid synthesis was up to eightfold increased and several intermediates of the pathway together with special cyclic carotenoids, beta-zeacarotene and 7,8-dihydro-beta-carotene, accumulated. All transformants with an over-expressed al-2 gene (encoding a phytoene synthase and a lycopene cyclase) displayed up to 31% prolonged life span.
在真菌嗜鸟粪层孔菌中已鉴定出类胡萝卜素，并建立了一条与八氢番茄红素和β-胡萝卜素平行的途径。已克隆了β-胡萝卜素分支的三个基因，并阐明了它们的功能。它们对应于粗糙脉孢菌的al-1、al-2和al-3基因。为了在定性和定量方面改变类胡萝卜素的组成，将它们单独或组合在嗜鸟粪层孔菌中过表达。在所得的转化体中，类胡萝卜素合成增加了高达八倍，该途径的几种中间体以及特殊的环状类胡萝卜素、β-玉米胡萝卜素和7,8-二氢-β-胡萝卜素积累。所有al-2基因（编码八氢番茄红素合酶和番茄红素环化酶）过表达的转化体寿命延长了31%。
