Windham G L, Williams W P
J Nematol. 1994 Mar;26(1):80-5.
Rates of penetration and development ofMeloidogyne incognita race 4 in roots of resistant (inbred Mp307, and S4 lines derived from the open-pollinated varieties Tebeau and Old Raccoon) and susceptible (Pioneer 3110) corn genotypes were determined. Seedlings grown in styrofoam containers were inoculated with 5,000 eggs of M. incognita. Roots were harvested at 3-day intervals starting at 3 days after inoculation (DAI) to 27 DAI and stained with acid fuchsin. Penetration of roots by second-stage juveniles (J2) at 3 DAI was similar for the four corn genotypes. Meloidogyne incognita numbers in Tebeau, Old Raccoon, Mp307, and Pioneer 3110 peaked at 12, 12, 15, and 27 DAI, respectively. Nematode development in the resistant genotypes was greatly suppressed compared to Pioneer 3110. Resistance to M. incognita in these genotypes appears to be expressed primarily as slower nematode development rather than differences in J2 penetration.
测定了南方根结线虫4号小种在抗性（自交系Mp307以及源自开放授粉品种Tebeau和Old Raccoon的S4品系）和敏感（先锋3110）玉米基因型根系中的侵入率和发育情况。在聚苯乙烯泡沫塑料容器中生长的幼苗接种了5000粒南方根结线虫卵。从接种后3天（DAI）开始，每隔3天收获根系，直至接种后27天，并使用酸性品红进行染色。在接种后3天，四个玉米基因型的根系被二龄幼虫（J2）侵入的情况相似。Tebeau、Old Raccoon、Mp307和先锋3110中的南方根结线虫数量分别在接种后12天、12天、15天和27天达到峰值。与先锋3110相比，抗性基因型中的线虫发育受到极大抑制。这些基因型对南方根结线虫的抗性似乎主要表现为线虫发育较慢，而非J2侵入的差异。
