Caglayan A O, Kalay N, Saatci C, Yalcýn A, Akalýn H, Dundar M
Department of Medical Genetics, Erciyes University Medical Faculty, Kayseri, Turkey.
Can J Cardiol. 2009 Mar;25(3):e69-72. doi: 10.1016/s0828-282x(09)70044-3.
Coronary endothelial dysfunction plays an important pathogenetic role in patients with slow coronary flow (SCF). No data exist regarding the possible contribution of the Glu298Asp polymorphism genotype of the endothelial nitric oxide synthase (eNOS) gene to human SCF in the literature.
To investigate the association between SCF and the Glu298Asp polymorphism of the eNOS gene.
The study population consisted of 85 consecutive patients. The patient group included 66 patients with angiographically proven normal coronary arteries with SCF, and 19 subjects with normal coronary arteries with no SCF. The thrombolysis in myocardial infarction frame count was used for the diagnosis of SCF. The Glu298Asp polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism.
The baseline characteristics were similar between the two groups, except for high-density lipoprotein cholesterol, which was higher in the SCF group than in the controls. The genotype distribution of Glu298Asp was as follows: GG 26%, GT 56% and TT 12%, where G is guanine and T is thymine. There was no difference in the frequency of the various genotypes or the alleles in patients with SCF versus normal controls.
The Glu298Asp polymorphism genotype of the eNOS gene is not a risk factor for SCF in the present study population.
冠状动脉内皮功能障碍在冠状动脉血流缓慢（SCF）患者的发病机制中起重要作用。文献中尚无关于内皮型一氧化氮合酶（eNOS）基因Glu298Asp多态性基因型对人类SCF可能影响的数据。
探讨SCF与eNOS基因Glu298Asp多态性之间的关联。
研究人群包括85例连续患者。患者组包括66例经血管造影证实冠状动脉正常但有SCF的患者，以及19例冠状动脉正常且无SCF的受试者。采用心肌梗死溶栓帧数来诊断SCF。通过聚合酶链反应和限制性片段长度多态性来确定Glu298Asp多态性。
两组的基线特征相似，但高密度脂蛋白胆固醇除外，SCF组的该指标高于对照组。Glu298Asp的基因型分布如下：GG占26%，GT占56%，TT占12%，其中G代表鸟嘌呤，T代表胸腺嘧啶。SCF患者与正常对照组相比，各种基因型或等位基因的频率没有差异。
在本研究人群中，eNOS基因的Glu298Asp多态性基因型不是SCF的危险因素。
