Sezgin Nurzen, Tekin Abdullah, Atac Fatma Belgin, Verdi Hasibe, Sezgin Alpay Turan
Department of Biochemistry, Acıbadem University School of Medicine, İstanbul, Turkey.
Department of Cardiology, Başkent University School of Medicine, Ankara, Turkey.
Interv Med Appl Sci. 2017 Sep;9(3):117-122. doi: 10.1556/1646.9.2017.17.
The aim of this study was to explore potential associations of the intron 4 variable number of tandem repeats (VNTR) and E298A polymorphisms of the endothelial nitric oxide synthase (eNOS) gene with slow coronary flow (SCF). The association between plasma nitrate and nitrite (NO ) concentrations and eNOS gene polymorphisms was also assessed.
The intron 4 VNTR and E298A polymorphisms of the eNOS gene were evaluated in the isolated DNA blood samples obtained from the SCF patient group ( = 30) and healthy group consisted of age- and sex-matched controls ( = 61).
Plasma NO level was significantly lower in patients with SCF than in controls. In addition, patients with SCF have significantly lower nitric oxide levels than control subjects within each genotype variants. The allele and genotyped frequencies of the eNOS intron 4 VNTR and E298A polymorphisms were similar between patients with SCF and the controls. Plasma NO concentrations with respect to the relevant genotypes were found insignificant.
Plasma NO is lower in patients with SCF than in healthy subjects. Our findings may suggest the lack of association between intron 4 VNTR and E298A polymorphisms of the eNOS gene and SCF.
本研究旨在探讨内皮型一氧化氮合酶（eNOS）基因第4内含子可变串联重复序列（VNTR）和E298A多态性与冠状动脉血流缓慢（SCF）之间的潜在关联。同时还评估了血浆硝酸盐和亚硝酸盐（NO ）浓度与eNOS基因多态性之间的关联。
在从SCF患者组（n = 30）和由年龄和性别匹配的对照组组成的健康组（n = 61）获得的分离DNA血样中评估eNOS基因的第4内含子VNTR和E298A多态性。
SCF患者的血浆NO 水平显著低于对照组。此外，在每种基因型变体中，SCF患者的一氧化氮水平均显著低于对照受试者。SCF患者和对照组之间eNOS第4内含子VNTR和E298A多态性的等位基因和基因型频率相似。发现血浆NO 浓度与相关基因型之间无显著关联。
SCF患者的血浆NO 水平低于健康受试者。我们的研究结果可能表明eNOS基因第4内含子VNTR和E298A多态性与SCF之间缺乏关联。
