Kuhlmann Ulrike C, Chwieralski Caroline E, Reinhold Dirk, Welte Tobias, Buhling Frank
Institute of Clinical and Molecular Immunology, Otto-von-Guericke-University-Magdeburg, Magdeburg.
Int J Radiat Biol. 2009 Feb;85(2):138-43. doi: 10.1080/09553000802641136.
Lung fibrosis can be caused by radiation therapy during cancer treatment and therefore can be the limiting factor of the treatment. The factors that cause the actual fibrosis and the interaction between different cell types were investigated.
Epithelial lung cells and fibroblasts were irradiated and different cytokines were measured in the supernatant. Also effects of radiation on the matrix production of fibroblasts were investigated.
Irradiation of isolated lung fibroblasts did not cause increased extracellular matrix production; however, the co-culturing of fibroblasts and irradiated lung epithelial cells or the treatment of fibroblasts with supernatants of irradiated epithelial cells did result in an increase. We were able to show that increased interleukin-8 (IL-8) levels led to increased matrix production.
IL-8 is not only a proinflammatory cytokine but it also stimulates collagen synthesis and matrix production and therefore could be a possible drug target in preventing radiation damage during cancer therapy.
肺癌治疗期间的放射治疗可导致肺纤维化，因此可能成为治疗的限制因素。本研究对引发实际纤维化的因素以及不同细胞类型之间的相互作用进行了调查。
对肺上皮细胞和成纤维细胞进行照射，并检测上清液中不同细胞因子的含量。同时研究了辐射对成纤维细胞基质产生的影响。
对分离的肺成纤维细胞进行照射不会导致细胞外基质产生增加；然而，成纤维细胞与受照射的肺上皮细胞共培养，或用受照射上皮细胞的上清液处理成纤维细胞，确实会导致细胞外基质产生增加。我们能够证明，白细胞介素-8（IL-8）水平升高会导致基质产生增加。
IL-8不仅是一种促炎细胞因子，还能刺激胶原蛋白合成和基质产生，因此可能是预防癌症治疗期间辐射损伤的潜在药物靶点。
