Beck Caroline W, Izpisúa Belmonte Juan Carlos, Christen Bea
Department of Zoology and Genetics Otago, University of Otago, New Zealand.
Dev Dyn. 2009 Jun;238(6):1226-48. doi: 10.1002/dvdy.21890.
While Xenopus is a well-known model system for early vertebrate development, in recent years, it has also emerged as a leading model for regeneration research. As an anuran amphibian, Xenopus laevis can regenerate the larval tail and limb by means of the formation of a proliferating blastema, the lens of the eye by transdifferentiation of nearby tissues, and also exhibits a partial regeneration of the postmetamorphic froglet forelimb. With the availability of inducible transgenic techniques for Xenopus, recent experiments are beginning to address the functional role of genes in the process of regeneration. The use of soluble inhibitors has also been very successful in this model. Using the more traditional advantages of Xenopus, others are providing important lineage data on the origin of the cells that make up the tissues of the regenerate. Finally, transcriptome analyses of regenerating tissues seek to identify the genes and cellular processes that enable successful regeneration. Developmental Dynamics 238:1226-1248, 2009. (c) 2009 Wiley-Liss, Inc.
非洲爪蟾是早期脊椎动物发育研究中一个广为人知的模式生物，近年来，它也成为再生研究的主要模式生物。作为一种无尾两栖动物，非洲爪蟾（Xenopus laevis）能够通过形成增殖性芽基来再生幼体的尾巴和肢体，通过附近组织的转分化来再生眼睛晶状体，并且还能使变态后的幼蛙前肢部分再生。随着非洲爪蟾诱导转基因技术的出现，最近的实验开始探讨基因在再生过程中的功能作用。在这个模式生物中，使用可溶性抑制剂也取得了很大成功。利用非洲爪蟾更传统的优势，其他研究正在提供有关构成再生组织的细胞起源的重要谱系数据。最后，对再生组织的转录组分析旨在识别能够实现成功再生的基因和细胞过程。《发育动力学》238:1226 - 1248，2009年。(c) 2009威利 - 利斯公司。
