Scholz Martin, Lipinski Marta, Leupold Marco, Luftmann Heinrich, Harig Lena, Ofir Rivka, Fischer Rainer, Prüfer Dirk, Müller Kai J
Westphalian Wilhelms-University Münster, Institute of Biochemistry and Biotechnology of Plants, Hindenburgplatz 55, 48143 Münster, Germany.
Phytochemistry. 2009 Mar;70(4):517-22. doi: 10.1016/j.phytochem.2009.01.018. Epub 2009 Mar 11.
Hydroponically cultivated Nigella sativa L. plants treated with methyl jasmonate (MeJA) showed a twelve-fold increase in levels of the monodesmosidic triterpene saponins alpha-hederin and kalopanaxsaponin I (KsI) in the leaves. We will demonstrate that these two saponins accounted for approximately 10% of the dry plant matter, of which 93% was KsI and 7% alpha-hederin. To address the molecular basis of saponin induction by MeJA, we cloned and characterized the beta-amyrin synthase gene (NsbetaAS1) encoding one of the key enzymes in triterpene saponin biosynthesis. As expected, NsbetaAS1 transcription was induced by MeJA and led to the production of beta-amyrin when over-expressed in yeast.
用茉莉酸甲酯（MeJA）处理的水培黑种草植物叶片中，单糖链三萜皂苷α-常春藤皂苷和刺楸皂苷I（KsI）的含量增加了12倍。我们将证明这两种皂苷约占植物干物质的10%，其中93%是KsI，7%是α-常春藤皂苷。为了探究MeJA诱导皂苷产生的分子基础，我们克隆并鉴定了β-香树脂醇合酶基因（NsbetaAS1），该基因编码三萜皂苷生物合成中的关键酶之一。正如预期的那样，NsbetaAS1转录受到MeJA的诱导，并且在酵母中过表达时会导致β-香树脂醇的产生。
