Prignano Francesca, Pescitelli Leonardo, Becatti Matteo, Di Gennaro Paola, Fiorillo Claudia, Taddei Niccolò, Lotti Torello
Department of Dermatological Sciences, University of Florence, Florence, Italy.
J Dermatol Sci. 2009 Jun;54(3):157-67. doi: 10.1016/j.jdermsci.2009.02.004. Epub 2009 Mar 17.
Vitiligo is a chronic acquired hypomelanotic disorder affecting 0.5-2% of the world's population. The two major pathogenetic hypotheses are focused on immune-mediated or toxic-mediated cell damage primarily directed at melanocytes. Recent experimental data underline the complex interactions that exist between melanocytes and other cells found in the skin.
Among these cells, keratinocytes are able to influence both the survival and the functional activity of melanocytes. In order to gain insights into the involvement of different types of epidermic cells in the pathogenesis of vitiligo, we have performed an ultrastructural study on lesional, perilesional and normal skin from 12 patients. All these patients suffered from non-segmental vitiligo, with a similar clinical history in terms of lesion extension and duration of the disease.
We have therefore grown cultures of keratinocytes from lesional, perilesional and healthy skin, evaluating the presence of oxidative damage and apoptotic markers in the cells.
Taken together, our results indicate that keratinocytes from perilesional skin show features of damaged cells.
Our data, besides considering the achromic patch as the terminal event of a chain of biological processes that take place in the perilesional skin, highlight keratinocytes as having an important role in the development of vitiligo.
白癜风是一种慢性获得性色素脱失性疾病，影响全球0.5%-2%的人口。两种主要的发病机制假说主要集中在免疫介导或毒性介导的细胞损伤，主要针对黑素细胞。最近的实验数据强调了黑素细胞与皮肤中其他细胞之间存在的复杂相互作用。
在这些细胞中，角质形成细胞能够影响黑素细胞的存活和功能活性。为了深入了解不同类型的表皮细胞在白癜风发病机制中的作用，我们对12例患者的皮损、皮损周边和正常皮肤进行了超微结构研究。所有这些患者均患有非节段性白癜风，在皮损范围和病程方面有相似的临床病史。
因此，我们培养了来自皮损、皮损周边和健康皮肤的角质形成细胞，评估细胞中氧化损伤和凋亡标志物的存在情况。
综合来看，我们的结果表明皮损周边皮肤的角质形成细胞呈现出受损细胞的特征。
我们的数据，除了将色素脱失斑视为皮损周边皮肤中发生的一系列生物过程的终末事件外，还强调角质形成细胞在白癜风发展中具有重要作用。
