Ohguro N, Matsuda M, Kinoshita S
Department of Ophthalmology, Osaka University Medical School, Japan.
Am J Ophthalmol. 1991 Oct 15;112(4):424-30. doi: 10.1016/s0002-9394(14)76252-x.
In an in vivo cat model, wide-field specular microscopy and scanning electron microscopy were used to observe whether heat-denatured sodium hyaluronate causes cell damage to the corneal endothelium. The endothelial cell toxicities of various drugs, including 0.01% benzalkonium chloride, 0.2% chlorhexidine digluconate, and 0.5% lidocaine, mixed with intact or untreated sodium hyaluronate were also investigated. Neither heat-denatured nor intact or untreated sodium hyaluronate alone had any adverse effect on the corneal endothelium in cats, whereas 0.01% benzalkonium chloride and 0.2% chlorhexidine digluconate, mixed with sodium hyaluronate, caused substantial endothelial morphologic changes, which resulted in corneal edema. By comparison, 0.5% lidocaine with sodium hyaluronate was found to have minimal effect on the corneal endothelium in cats. These findings indicate that some chemical contaminant with sodium hyaluronate, not heat-denatured sodium hyaluronate, induces an immediate onset of pseudophakic bullous keratopathy.
在一个体内猫模型中，使用广角镜面显微镜和扫描电子显微镜观察热变性透明质酸钠是否会对角膜内皮细胞造成损伤。还研究了包括0.01%苯扎氯铵、0.2%葡萄糖酸氯己定和0.5%利多卡因在内的各种药物与完整或未处理的透明质酸钠混合后的内皮细胞毒性。单独的热变性透明质酸钠、完整或未处理的透明质酸钠对猫的角膜内皮均无任何不良影响，而0.01%苯扎氯铵和0.2%葡萄糖酸氯己定与透明质酸钠混合后，会引起明显的内皮形态学改变，进而导致角膜水肿。相比之下，0.5%利多卡因与透明质酸钠混合后对猫的角膜内皮影响极小。这些发现表明，与透明质酸钠混合的某些化学污染物而非热变性透明质酸钠会导致人工晶状体性大泡性角膜病变立即发作。
